Protein Info for PFLU_RS00385 in Pseudomonas fluorescens SBW25
Annotation: homoserine kinase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 74% identical to KHSE_PSEAB: Homoserine kinase (thrB) from Pseudomonas aeruginosa (strain UCBPP-PA14)
KEGG orthology group: K02204, homoserine kinase type II [EC: 2.7.1.39] (inferred from 100% identity to pfs:PFLU0077)Predicted SEED Role
"Homoserine kinase (EC 2.7.1.39)" in subsystem Methionine Biosynthesis or Threonine and Homoserine Biosynthesis (EC 2.7.1.39)
MetaCyc Pathways
- aspartate superpathway (22/25 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- superpathway of L-threonine biosynthesis (6/6 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- L-threonine biosynthesis (2/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.39
Use Curated BLAST to search for 2.7.1.39
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K6B1 at UniProt or InterPro
Protein Sequence (317 amino acids)
>PFLU_RS00385 homoserine kinase (Pseudomonas fluorescens SBW25) MSVFTPLARPELETFLAPYGLGRLIDFQGIAAGSENTNFFISLEQGEFVLTLVERGPVAE MPFFIELLDVLHDADLPVPYALRTTDGVALRELKGKPALLQPRLAGKHIKDANAQHCAQV GDLLGHLHLATQGEKVLERKTDRGLDWMLNEGAQLISHLNDAQQRLLQDALNEIKAHKAQ ILALPRANVHADLFRDNAMFEGTHLTGLIDFYNACSGPMLYDVAIALNDWCSDADGVIDG QRARALLGAYAGLRPFTAKEAELWPTMLRVACVRFWLSRLIAAESFAGQDVLIHDPAEFE HRLAQRQQVTIQLPFAL