Protein Info for PFLU_RS00165 in Pseudomonas fluorescens SBW25

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 transmembrane" amino acids 20 to 35 (16 residues), see Phobius details amino acids 56 to 75 (20 residues), see Phobius details amino acids 88 to 115 (28 residues), see Phobius details amino acids 147 to 168 (22 residues), see Phobius details amino acids 174 to 195 (22 residues), see Phobius details amino acids 246 to 266 (21 residues), see Phobius details amino acids 284 to 307 (24 residues), see Phobius details amino acids 319 to 337 (19 residues), see Phobius details amino acids 343 to 364 (22 residues), see Phobius details amino acids 375 to 398 (24 residues), see Phobius details amino acids 405 to 426 (22 residues), see Phobius details PF07690: MFS_1" amino acids 26 to 392 (367 residues), 199.3 bits, see alignment E=4.4e-63 TIGR00893: D-galactonate transporter" amino acids 28 to 426 (399 residues), 420 bits, see alignment E=4.7e-130

Best Hits

Swiss-Prot: 43% identical to GUDP_ECOLI: Probable glucarate transporter (gudP) from Escherichia coli (strain K12)

KEGG orthology group: K08194, MFS transporter, ACS family, D-galactonate transporter (inferred from 100% identity to pfs:PFLU0033)

MetaCyc: 43% identical to galactarate/D-glucarate transporter GudP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-203; TRANS-RXN0-204; TRANS-RXN0-523

Predicted SEED Role

"D-galactonate transporter" in subsystem D-galactonate catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K566 at UniProt or InterPro

Protein Sequence (436 amino acids)

>PFLU_RS00165 MFS transporter (Pseudomonas fluorescens SBW25)
MHPESFTGQASLVTPTRKRFFIMVLLFITVVINYLDRSNLSIAAPALTSELGIDPVHVGL
IFSAFGWTYAAMQIPGGWLVDRVPPRILYTAALLLWSIATVMLGFAASFIALFVLRMAVG
ALEAPAYPINSRVVTTWFPERERATAIGVYTSGQFVGLAFLTPVLAWLQHAFGWHMVFVA
TGGVGILWAVIWYAVYREPKDFKGANAAEIELIREGGGLVDMQAKTVKAPFSWVDLGIVL
SKRKLWGIYLGQFCLNSTLWFFLTWFPTYLVKYRGMDFIKSGLLASLPFLAAFVGVLCSG
VFSDWLIRRGASVGFARKLPIIGGLLISTAIIGANYVDSTAWVIAFLAVAFFGNGLASIT
WSLVSTLAPARLLGLTGGVFNFIGNLAAITTPIVIGFLASGDSFAPAITYIAVLALLGAL
SYVLLVGKVERIELNE