Protein Info for PFLU_RS00100 in Pseudomonas fluorescens SBW25

Annotation: DNA-protecting protein DprA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 transmembrane" amino acids 234 to 246 (13 residues), see Phobius details TIGR00732: DNA protecting protein DprA" amino acids 79 to 296 (218 residues), 260.6 bits, see alignment E=4.2e-82 PF02481: DNA_processg_A" amino acids 84 to 288 (205 residues), 253.1 bits, see alignment E=1.6e-79 PF17782: DprA_WH" amino acids 313 to 358 (46 residues), 24.7 bits, see alignment 2e-09

Best Hits

KEGG orthology group: K04096, DNA processing protein (inferred from 100% identity to pfs:PFLU0020)

Predicted SEED Role

"Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4H7 at UniProt or InterPro

Protein Sequence (364 amino acids)

>PFLU_RS00100 DNA-protecting protein DprA (Pseudomonas fluorescens SBW25)
MNPINCHEISPSELEARLRLHRLPELGPKRFRLLIEAFGSASKAISAPASAWRSLGVPAI
SADARRSHEVRDGASAALAWLACQGQHLLMWDQPDYPALLAQIDDAPPLLFVAGDPLILE
KPQLAMVGSRRASRPGMDTAAAFSRSLASAGFVITSGLALGIDGAAHQAALDVGGQTIGV
LGTGLENFYPQRHKRLAAAMIDQGSAVISEFPLDAPPQAGNFPRRNRIISGLSLGVLVVE
ASMASGSLITAKLAAEQGREVYAIPGSIHHPGAKGCHQLIRDGAVLVETIEHILEGLRGW
QALSRPTPMPVVHPLVALLHAAPHTSEGLAIASGRSLSHVLATLTELELEGQVICESGRW
LARC