Protein Info for OLJFJH_16950 in Erwinia amylovora T8

Annotation: 2-oxoglutarate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16078: 2-oxogl_dehyd_N
  • amino acids 12 to 49 (38 residues), 55.1 bits, see alignment (E = 9e-19)
• TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component
  • amino acids 15 to 932 (918 residues), 1578.6 bits, E=0
• PF00676: E1_dh
  • amino acids 226 to 516 (291 residues), 149.5 bits, E=2.2e-47
• PF02779: Transket_pyr
  • amino acids 592 to 785 (194 residues), 176.6 bits, E=7.3e-56
• PF16870: OxoGdeHyase_C
  • amino acids 790 to 932 (143 residues), 183.9 bits, E=3.2e-58

Best Hits

Swiss-Prot: 88% identical to ODO1_ECO57: 2-oxoglutarate dehydrogenase E1 component (sucA) from Escherichia coli O157:H7

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 100% identity to eay:EAM_1172)

MetaCyc: 88% identical to subunit of E1(0) component of 2-oxoglutarate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Oxoglutarate dehydrogenase (succinyl-transferring). [EC: 1.2.1.105, 1.2.4.2]

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• L-tryptophan degradation III (eukaryotic) (3/15 steps found)
• superpathway of L-lysine degradation (10/43 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Lysine degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.105 or 1.2.4.2

Protein Sequence (935 amino acids)

>OLJFJH_16950 2-oxoglutarate dehydrogenase E1 component (Erwinia amylovora T8)
MQNSAMKPWLDSSWLAGANQSYIEQLYEDFLTDPDSVDVAWKSLFQQLPGTGVRPEQIHS
TTREYFRRLAKDASRYNTPISDPATNAKQVKVLQLINAFRFRGHQQANLDPLGLWKQEPV
ADLDPGYHGLNEADFQESFNVGSFAIGKETMKLADLYAALKQTYCGSIGAEYMHITNTDE
KRWIQQRLESVVGQASYSVEEKKGFLRQLTAAEGLERYLGAKFPGAKRFSLEGGDALVPM
LNEMIRHAGKSGTREVVLGMAHRGRLNVLINVLGKKPQDLFDEFSGKHKEHLGTGDVKYH
MGFSSDVETEGGMVHLALAFNPSHLEIVSPVVMGSVRARLDRLDQPGSNKVLPITIHGDA
AVIGQGVVQETLNMSEARGYEVGGTVRIVINNQIGFTTSNPKDARSTQYCTDIGKMVMAP
IFHVNADDPEAVAFVTRLALDFRNTFKRDVFIDLVCYRRHGHNEADEPSATQPVMYQKIK
KHPTPRKIYADKLEAGQVASLEDATEMVNLYRDALDEGECVVPEWRPMSLHSFTWSPYLN
HDWDESYPERIELKRLQELAKRISTVPEAVEMQSRVTKIYNDRADMAAGNKPFDWGAAEN
LAYATLVDEGIPCRLSGEDMGRGTFFHRHAVIHNQSNGSTYTPLQHIHNGQGTFKVWDSV
LSEEAVLAFEYGYATAEPRTLTIWEAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLL
PHGYEGQGPEHSSARLERYLQLCAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSP
KSLLRHPLAVSSLEELANGAFQPAIGEIDALDAKSVKRVVLCSGKVYYDLLDKRRKNEQT
DVAIVRIEQLYPFPHKAVQDVLKQYSHVHDFVWCQEEPLNQGAWYCSQHHFREIVPFGAS
LRYAGRPASASPAVGYMSVHQQQQQDLVNDALNVE