Protein Info for OLJFJH_16415 in Erwinia amylovora T8

Annotation: EmrB/QacA family drug resistance transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 54 to 74 (21 residues), see Phobius details amino acids 82 to 101 (20 residues), see Phobius details amino acids 107 to 129 (23 residues), see Phobius details amino acids 141 to 162 (22 residues), see Phobius details amino acids 168 to 188 (21 residues), see Phobius details amino acids 206 to 225 (20 residues), see Phobius details amino acids 237 to 258 (22 residues), see Phobius details amino acids 275 to 298 (24 residues), see Phobius details amino acids 306 to 330 (25 residues), see Phobius details amino acids 340 to 357 (18 residues), see Phobius details amino acids 374 to 394 (21 residues), see Phobius details amino acids 486 to 512 (27 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 16 to 506 (491 residues), 429.9 bits, see alignment E=6.3e-133 PF00083: Sugar_tr" amino acids 18 to 195 (178 residues), 48.1 bits, see alignment E=1.3e-16 PF07690: MFS_1" amino acids 21 to 417 (397 residues), 182 bits, see alignment E=2.3e-57 PF06609: TRI12" amino acids 35 to 297 (263 residues), 23.9 bits, see alignment E=2.1e-09

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 100% identity to eam:EAMY_1280)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (522 amino acids)

>OLJFJH_16415 EmrB/QacA family drug resistance transporter (Erwinia amylovora T8)
MSSGQSWKPASNPWLVAMTVTLAVFMEILDTTIVNVALPHVAGSLSASYDESTWVLTSYL
VANGIVLPISAFLSRLLGRKQFFLVCIVMFTVCSFLCGIATELWQIILFRVLQGFFGGGL
QPVQQSVLLDYFKPADRGKAFGLSSIAVIVAPVLGPTLGGWITDNYSWRWVFFINIPVGI
FSVLAIYQLLEDPPWERKLAKGKLSIDYIGIGLITLGLGCLQVMMDRGEDADWFQSSFIV
TFALLALTGIVGAVYWLMYAKRPVVDLLVLKDRNFAVACLLMAGMAVILYGSSVVIPQLA
QQDLGYTATLSGLVLSPGAVLIVLSIPLVLKLMPLVQTRLLIAFGFTLLGVSFIYSSTLT
PDIDFTTLVLMRSFQTIGLGFLFVPLTTIAFVTIPQRLNADASALFTMFRNVAGSIGISL
STSMITERSQARSAQLADGMTPFNEPFNLTLERWTQGVRDFAHTAGDPLTIASGQLYKQM
IVQARFLAYIDVFMGLSIVALILIPFCLLLAPIKSEGSAGAH