Protein Info for OLJFJH_14855 in Erwinia amylovora T8

Annotation: PTS sucrose transporter subunit IIBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 transmembrane" amino acids 108 to 132 (25 residues), see Phobius details amino acids 144 to 166 (23 residues), see Phobius details amino acids 177 to 197 (21 residues), see Phobius details amino acids 207 to 230 (24 residues), see Phobius details amino acids 245 to 268 (24 residues), see Phobius details amino acids 287 to 308 (22 residues), see Phobius details amino acids 328 to 350 (23 residues), see Phobius details amino acids 361 to 381 (21 residues), see Phobius details amino acids 389 to 410 (22 residues), see Phobius details amino acids 428 to 449 (22 residues), see Phobius details TIGR01996: PTS system, sucrose-specific IIBC component" amino acids 3 to 448 (446 residues), 640.5 bits, see alignment E=2.1e-196 PF00367: PTS_EIIB" amino acids 9 to 40 (32 residues), 47.4 bits, see alignment (E = 1e-16) TIGR00826: PTS system, glucose-like IIB component" amino acids 28 to 111 (84 residues), 82.7 bits, see alignment E=2.8e-27 PF02378: PTS_EIIC" amino acids 110 to 395 (286 residues), 186.4 bits, see alignment E=7.5e-59 TIGR00852: PTS system, maltose and glucose-specific subfamily, IIC component" amino acids 153 to 437 (285 residues), 301.9 bits, see alignment E=7.2e-94

Best Hits

Swiss-Prot: 81% identical to PTSBC_KLEPN: PTS system sucrose-specific EIIBC component (scrA) from Klebsiella pneumoniae

KEGG orthology group: K02809, PTS system, sucrose-specific IIB component [EC: 2.7.1.69] K02810, PTS system, sucrose-specific IIC component (inferred from 100% identity to eay:EAM_1596)

MetaCyc: 81% identical to Enzyme IIscr (Klebsiella pneumoniae)
SUCROSEPHOSPHO-RXN [EC: 2.7.1.211]

Predicted SEED Role

"PTS system, sucrose-specific IIB component (EC 2.7.1.69) / PTS system, sucrose-specific IIC component (EC 2.7.1.69)" in subsystem Sucrose utilization (EC 2.7.1.69)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.211 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>OLJFJH_14855 PTS sucrose transporter subunit IIBC (Erwinia amylovora T8)
VNIEKTARELIPLLGGRENIASAAHCATRLRLVMADDEKVQKEAIEALEGVKGCFRNAGQ
MQIIFGSGIVNKVHAAFVREAGIGESSKSEVADIAAKKLNPLQRIARVLSNIFVPIIPAI
VASGLLMGLLGMVKTYGWVGSGSALFIMLDMCSSAAFIILPVLIGFTAAREFGGNPYLGA
TLGGILTHPALTNAWGVASGFHTMNFFGLEIAMIGYQGTVFPVLLAVWFMSHCEKRLRQI
IPDALDIILTPFLTVIISGFVALLIIGPAGRAFGDGLSLVLSTLIGHAGWLAGLLFGGLY
SVIVITGVHHSFHAVEAGLLGNPSIGVNFLLPIWSMANVAQGGACLAVWFKTRDVKVRSI
VVPSGFSALLGITEAAIFGVNLRYIKPFIAGLAGGALGGAWVVSMHVNMTAVGLTALPGM
AIVQASSLVNYLIGMVIAFTTAFVLSLLLKIKFEVKA