Protein Info for OLJFJH_07115 in Erwinia amylovora T8

Annotation: outer membrane lipid asymmetry maintenance protein MlaD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 182 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR04430: outer membrane lipid asymmetry maintenance protein MlaD" amino acids 2 to 152 (151 residues), 174.2 bits, see alignment E=7e-56 PF02470: MlaD" amino acids 39 to 115 (77 residues), 72.3 bits, see alignment E=1.6e-24

Best Hits

Swiss-Prot: 68% identical to MLAD_ECO57: Intermembrane phospholipid transport system binding protein MlaD (mlaD) from Escherichia coli O157:H7

KEGG orthology group: K02067, putative ABC transport system substrate-binding protein (inferred from 100% identity to eay:EAM_3095)

Predicted SEED Role

"Uncharacterized ABC transporter, periplasmic component YrbD"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (182 amino acids)

>OLJFJH_07115 outer membrane lipid asymmetry maintenance protein MlaD (Erwinia amylovora T8)
MQSKKSEIWVGAFLLLALCAIIFLCLRVTDLKSFGNVSTWKLYATFDNVGGLKPGSPVKV
GGVVIGRVSDITLDAKSYSPKVSMDIDERYNNIPDTSSLAVRTSGLLGEQYLALNIGFND
PEMGTALLKNGGTVQDTKSAMVLEDLIGQFLYKSGNNDEKNPPAEGDKAAQGQVAPAAAN
NP