Protein Info for OLJFJH_04030 in Erwinia amylovora T8

Annotation: bifunctional succinylornithine transaminase/acetylornithine transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03246: succinylornithine transaminase family
  • amino acids 8 to 403 (396 residues), 677.3 bits, E=7e-208
• TIGR00707: transaminase, acetylornithine/succinylornithine family
  • amino acids 17 to 400 (384 residues), 511.3 bits, E=1.4e-157
• PF00202: Aminotran_3
  • amino acids 25 to 400 (376 residues), 464.7 bits, E=2.2e-143
• PF00155: Aminotran_1_2
  • amino acids 155 to 325 (171 residues), 25 bits, E=1.1e-09

Best Hits

Swiss-Prot: 79% identical to ARGD_ECOL6: Acetylornithine/succinyldiaminopimelate aminotransferase (argD) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00821, acetylornithine/N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.11 2.6.1.17] (inferred from 100% identity to eay:EAM_3227)

MetaCyc: 79% identical to N-acetylornithine aminotransferase / N-succinyldiaminopimelate aminotransferase (Escherichia coli K-12 substr. MG1655)
Succinyldiaminopimelate transaminase. [EC: 2.6.1.17]; Acetylornithine transaminase. [EC: 2.6.1.17, 2.6.1.11]

Predicted SEED Role

"Acetylornithine aminotransferase (EC 2.6.1.11) / N-succinyl-L,L-diaminopimelate aminotransferase (EC 2.6.1.17)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway (EC 2.6.1.11, EC 2.6.1.17)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• aspartate superpathway (21/25 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.17

Protein Sequence (405 amino acids)

>OLJFJH_04030 bifunctional succinylornithine transaminase/acetylornithine transaminase (Erwinia amylovora T8)
MAAEKIAVTRATFDEVILPVYAPAQFVPVKGKGSRIWDQQGKEYIDFSGGIAVTALGHCH
PALVEALKTQGETLWHTSNVFTNEPALRLARKLIDATFAERVFFANSGAEANEAAFKLAR
YYASQRHSPYKSKIIAFHNAFHGRTLFTVSVGGQPKYSDGFGPKPADIVHVPFNDLAAVK
AVIDDHTCAIVVEPIQGEGGVVPATEEFMQGLRQLCDEHHALLVMDEVQSGMGRSGKLFA
YEHYGVQPDIVTSAKALGGGFPVSAMLTTNAIASVMAPGVHGTTYGGNPLACAVAEAALD
IINTPEVLDGVAARRQLFVDALQSLNTRRDLFSDIRGKGLLIGAALKPQYADKARDILNA
AAAEGVMVLVAGSGVMRFAPSLLIEPADIAEGMERFAAAVEKVLA