Protein Info for OLJFJH_03955 in Erwinia amylovora T8

Annotation: glutathione-regulated potassium-efflux system protein KefB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 32 to 50 (19 residues), see Phobius details amino acids 56 to 75 (20 residues), see Phobius details amino acids 87 to 108 (22 residues), see Phobius details amino acids 114 to 135 (22 residues), see Phobius details amino acids 148 to 171 (24 residues), see Phobius details amino acids 183 to 201 (19 residues), see Phobius details amino acids 213 to 231 (19 residues), see Phobius details amino acids 237 to 255 (19 residues), see Phobius details amino acids 268 to 288 (21 residues), see Phobius details amino acids 294 to 313 (20 residues), see Phobius details amino acids 326 to 349 (24 residues), see Phobius details amino acids 356 to 373 (18 residues), see Phobius details TIGR00932: transporter, monovalent cation:proton antiporter-2 (CPA2) family" amino acids 14 to 283 (270 residues), 295.4 bits, see alignment E=2.2e-92 PF00999: Na_H_Exchanger" amino acids 17 to 373 (357 residues), 213 bits, see alignment E=6.4e-67 PF02254: TrkA_N" amino acids 403 to 514 (112 residues), 87 bits, see alignment E=1.2e-28

Best Hits

Swiss-Prot: 96% identical to KEFB_ERWT9: Glutathione-regulated potassium-efflux system protein KefB (kefB) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K11747, glutathione-regulated potassium-efflux system protein KefB (inferred from 100% identity to eay:EAM_3212)

MetaCyc: 82% identical to K+ : H+ antiporter KefB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-42

Predicted SEED Role

"Glutathione-regulated potassium-efflux system protein KefB" in subsystem Glutathione-regulated potassium-efflux system and associated functions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (601 amino acids)

>OLJFJH_03955 glutathione-regulated potassium-efflux system protein KefB (Erwinia amylovora T8)
MEGQSLLTAGLLYLCAAVIAVPIAARLGIGAVLGYLLAGIAIGPWGLGFISDVQEILHFS
EMGVVFLMFIIGLELKPSKLWELRRSIFGVGAAQVLLSAAVLGGILWLTDFSWQAATIGG
IGLAMSSTAMALQLMRDKAMNRNESGQLGFSVLLFQDLAVIPALALVPLLAGSDSGHTDW
MKLGMKVLAFAGLLVGGRYLLRPIFRFIAASGVREVFTAAALLLVLGFALFMDALGLSMA
LGTFIAGILLAESEYRHELEIAIDPFKGLLLGLFFISVGMALNLGVLYTHMTEILTGVLL
LVAVKTAVLYLLARVYGLRSSERLQFSGVLSQGGEFAFVLFSAASSANLFSGDQMPLLLV
TVTLSMMTTPLLMQGVDKILRHRFNEVDDNQEKPFVEDDKPQVIVVGFGRFGQVVARLLM
ANERRITVLERDISAVSLMRRYGYKVYYGDATQLELLRAAGAETAQSLVITCNGPEDAMA
IVHLCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLMSLGMHPHQ
AFRAQQHFRRLDMRMLRELMPNHDDSQQISRVKEARRELEEIFQAELRHEKRQFDGWDDA
D