Protein Info for OLJFJH_03585 in Erwinia amylovora T8

Annotation: diacylglycerol kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 121 transmembrane" amino acids 30 to 51 (22 residues), see Phobius details amino acids 56 to 79 (24 residues), see Phobius details amino acids 98 to 118 (21 residues), see Phobius details PF01219: DAGK_prokar" amino acids 16 to 117 (102 residues), 122.2 bits, see alignment E=3.7e-40

Best Hits

Swiss-Prot: 77% identical to KDGL_ECOLI: Diacylglycerol kinase (dgkA) from Escherichia coli (strain K12)

KEGG orthology group: K00901, diacylglycerol kinase [EC: 2.7.1.107] (inferred from 99% identity to eay:EAM_0263)

MetaCyc: 77% identical to diacylglycerol kinase (Escherichia coli K-12 substr. MG1655)
Diacylglycerol kinase. [EC: 2.7.1.107]

Predicted SEED Role

"Diacylglycerol kinase (EC 2.7.1.107)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.1.107)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.107

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (121 amino acids)

>OLJFJH_03585 diacylglycerol kinase (Erwinia amylovora T8)
MANNATGLTRILKAAGYSWKGLRAAWRHEAAFRQEAAAAILAVVIAGLLDVDAITRVLLI
GSVILVIVVEILNSAIEAVVDRIGNEIHPLAGRAKDMGSAAVLITLLLAVFVWIMLLVPR
L