Protein Info for OLJFJH_03355 in Erwinia amylovora T8

Annotation: murein hydrolase effector protein LrgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 232 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 31 to 51 (21 residues), see Phobius details amino acids 63 to 83 (21 residues), see Phobius details amino acids 91 to 112 (22 residues), see Phobius details amino acids 149 to 169 (21 residues), see Phobius details amino acids 204 to 229 (26 residues), see Phobius details PF04172: LrgB" amino acids 14 to 224 (211 residues), 233.8 bits, see alignment E=7.3e-74

Best Hits

Swiss-Prot: 36% identical to YOHK_SHIFL: Inner membrane protein YohK (yohK) from Shigella flexneri

KEGG orthology group: None (inferred from 100% identity to eam:EAMY_3279)

Predicted SEED Role

"CidA-associated membrane protein CidB" in subsystem Murein hydrolase regulation and cell death

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (232 amino acids)

>OLJFJH_03355 murein hydrolase effector protein LrgB (Erwinia amylovora T8)
MSDFVIGILCLLATLVVYFCNKHLYRRWRRLVLMPLVMTPMVLVALLVFTRISWQDYIGE
SRWLLWLLGPATLAFAVPLYENLAIVRRHWLSLSAGVFTATLVAVCSSVWLARLLTLPEA
VQRSLAVRSITTPFALAAAKQVGGQPDLVALFVVITGVFGMATGDLLFLRLAIKQGVAKG
AGLGAASHGAGTARAYEIGQQEGVVSSLVMMLSGVVTVMIAPAIGHMMWATS