Protein Info for OLJFJH_03300 in Erwinia amylovora T8

Annotation: GGDEF domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 818 TIGR00229: PAS domain S-box protein" amino acids 19 to 126 (108 residues), 34.3 bits, see alignment E=2.3e-12 amino acids 130 to 255 (126 residues), 63 bits, see alignment E=3e-21 amino acids 276 to 393 (118 residues), 35.4 bits, see alignment E=1.1e-12 PF13426: PAS_9" amino acids 19 to 119 (101 residues), 28.6 bits, see alignment E=5.2e-10 amino acids 146 to 248 (103 residues), 48.1 bits, see alignment E=4.3e-16 amino acids 286 to 388 (103 residues), 20.6 bits, see alignment E=1.6e-07 PF00989: PAS" amino acids 136 to 240 (105 residues), 43.8 bits, see alignment E=8.5e-15 amino acids 276 to 386 (111 residues), 28.3 bits, see alignment E=5.5e-10 PF08448: PAS_4" amino acids 283 to 390 (108 residues), 25.7 bits, see alignment E=3.9e-09 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 397 to 559 (163 residues), 136 bits, see alignment E=1.1e-43 PF00990: GGDEF" amino acids 400 to 557 (158 residues), 162.9 bits, see alignment E=1.9e-51 PF00563: EAL" amino acids 577 to 808 (232 residues), 255.2 bits, see alignment E=1.8e-79

Best Hits

KEGG orthology group: None (inferred from 100% identity to eam:EAMY_3268)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (818 amino acids)

>OLJFJH_03300 GGDEF domain-containing protein (Erwinia amylovora T8)
MNEDSDVVYQLLARSEGNCAIYMLDPEGIVASWSLGAQHAKGYLPAEIIGRNFSCFYSQQ
DQMLGKPRHGLQVAREAGRFATKGWRYRKDGSAFWAHEVIDAVFDDNGKLLGYAKITRNC
TGQLQQDRQQRDQTFRLLVEGINDYAIYMLDLNGLVLNWNAGACRAKGFTCNEIIGKHFS
CFYSEQDRRHNLPDKNLQLATATGHFEDNGWRYRKDGSAFWAHVVIDAIRNDGGELIGFA
KITRDCTEMREYERQIINAKDLAEKSSKKMASLSQFLDSIVANIPSCVIVEDAVSGEILL
INNRAEQLLGGSQCEFIGKKAQECMTPAMSAYFMQLSDAAQRKGGVQRNERQLATSGGDR
IISASASMVRGKDARHSYVLLIADDVTDQRAADARIHHMAHHDNLTSLPNRVLFNQHLTT
ALREDRDAQRLTAVLGLDLDNFKNVNDALGHQVGDALLCSVAVRLRSVLRNRDTLARNGG
DEFSIVLPGLSHPEEAEAMARRLIETIRPPFTVHGHNLSIGLSIGITLAQHGMTSLDYLL
RCADMALYAAKRNGRNRFEHFTKAMGDTAQKRRIIENDLREAITYRQLKLYYQPITNNQH
REIIGYEALMRWHHPEKGIIMPLEFIPIAEETGLIHSLGTYALYEACREAASWPGEQTVA
VNLSPLQFKNSALIAVVEGALKESGLAPHRLEVEITESVLLDNTLANIDILRELKALGVQ
IALDDFGTGYSSLSYLRSFPFDKIKIDKSFINDMQDSREALAIIRAITGMSRSLDIQITA
EGVESNEQFQRLKAEGCTLFQGYYFGRPQPPERRLKEF