Protein Info for OLJFJH_02625 in Erwinia amylovora T8

Annotation: protein-disulfide reductase DsbD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 571 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 174 to 200 (27 residues), see Phobius details amino acids 213 to 237 (25 residues), see Phobius details amino acids 249 to 271 (23 residues), see Phobius details amino acids 293 to 323 (31 residues), see Phobius details amino acids 335 to 357 (23 residues), see Phobius details amino acids 369 to 387 (19 residues), see Phobius details amino acids 393 to 411 (19 residues), see Phobius details amino acids 418 to 437 (20 residues), see Phobius details PF11412: DsbD_N" amino acids 33 to 142 (110 residues), 112.4 bits, see alignment E=4e-36 PF02683: DsbD_TM" amino acids 175 to 387 (213 residues), 262.2 bits, see alignment E=9.2e-82 PF13386: DsbD_2" amino acids 176 to 367 (192 residues), 37 bits, see alignment E=1.1e-12 PF13098: Thioredoxin_2" amino acids 472 to 566 (95 residues), 35 bits, see alignment E=4.8e-12 PF13899: Thioredoxin_7" amino acids 473 to 543 (71 residues), 30.3 bits, see alignment E=1.1e-10

Best Hits

Swiss-Prot: 65% identical to DSBD_CROS8: Thiol:disulfide interchange protein DsbD (dsbD) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 100% identity to eay:EAM_0414)

MetaCyc: 64% identical to thiol-disulfide exchange protein DsbD (Escherichia coli K-12 substr. MG1655)
RXN-19949 [EC: 1.8.4.16]; 1.8.4.16 [EC: 1.8.4.16]; 1.8.4.16 [EC: 1.8.4.16]; RXN0-7311 [EC: 1.8.4.16]

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.8

Use Curated BLAST to search for 1.8.1.8 or 1.8.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (571 amino acids)

>OLJFJH_02625 protein-disulfide reductase DsbD (Erwinia amylovora T8)
MVQRITPFIFLLLTTLFNLNASATPFGQNNTGQFVTVNQAFAFDFSQKNDRLTLSWQVKP
GYYLYRQQIKISLKGATLARWSLPAGHPHEDEFYGKTAVYPDNLTLPFTLTQAAAGAQLT
VSYQGCAAAGFCYPPETRNVPLNAVVAQSSPANAAMPASSLKDSQEHSLPFSTLWALLIG
IGVAFTPCVLPMYPLISAIILGGNRQYSLGRLFALAMFYVQGMALTYTLMGLVVAAAGLR
FQAALQAPAVLIVLSALFILLALSMFGLFNLQLPPALQTRLTLWSNRQQGGSLTGVFLMG
ALAGLICSPCTTAPLSAILLYIAQSGNMLTGAATLYLYALGMGLPLIAITLFGNRLLPKN
GPWMQTVKEGFGFVILALPVFLLERVVGEIWGMRLWSGLCVAFFGWAFITSLKSNRGWLR
VLQVLLLGAMLITSRPLQDWAFGNAQGPQAAAPISFKPVTHVELLNQQLQNSTGKITMLD
LYADWCVACKEFDKYTFSDARVRAALNPMQLLQADVTANNSRDSALLKHLNVLGLPSILF
FAADGHEIPDSRITGFMKPSDFHAHLQKLTQ