Protein Info for OLJFJH_02515 in Erwinia amylovora T8

Annotation: N-acetylmuramoyl-L-alanine amidase AmiB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 552 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF11741: AMIN" amino acids 27 to 112 (86 residues), 29.5 bits, see alignment E=1.3e-10 PF01520: Amidase_3" amino acids 185 to 404 (220 residues), 161.3 bits, see alignment E=5e-51 PF01476: LysM" amino acids 449 to 491 (43 residues), 53.2 bits, see alignment 4.5e-18 amino acids 508 to 550 (43 residues), 48.2 bits, see alignment 1.7e-16

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 100% identity to eam:EAMY_3160)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (552 amino acids)

>OLJFJH_02515 N-acetylmuramoyl-L-alanine amidase AmiB (Erwinia amylovora T8)
MMRRLMCCWLLALFCCSFAAVAANLSDINVSNGSNEAQITLSFAGQPVYAFFPLHNPERV
VLDIRQSAIVRGLPLDFSGDNIVKRIRASTAKDSQSIRLVFDLTRRGKTRAVTRRDGRYK
VVFTITGNQPALAASRSVNASGNVISRAASSPKSPFDEDRVTRVSSGDEVARPRRARSSN
DTVIVAIDAGHGGQDPGAVGGGGLKEKNVTIAIARKLRALLNADPMFRGVMTREGDYFIS
VMGRSDVARKQNANLLVSIHADAAPARNASGASVWVLSNRRANSEMAGWLEQHEKQSELL
GGAGDLLANSQMDPYLSQAVLDLQFGHSQRVGYDVAIKVIGQLQRVGALHKRRPEHASLG
VLRSPDIPSLLVETGFISNPSEERLLGSSAYQQKIAESIYKGLRNYFLAHPLQSFPKEEN
RPLQPAAAVEPEADPPAASLSYAGASQRHVVKRGETLSGIAAHYGVSMATLRGMNHLKND
VVWVGQRLKVPAASKVASVAKREKSVKHKVVSGDSLTAIAAHYGVSPQAVQQANKLKSQN
VMLGQTLTIPTS