Protein Info for OLJFJH_01890 in Erwinia amylovora T8

Annotation: DotU family type VI secretion system protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 transmembrane" amino acids 201 to 222 (22 residues), see Phobius details TIGR03349: type IV/VI secretion system protein, DotU family" amino acids 21 to 229 (209 residues), 230.9 bits, see alignment E=1.4e-72 PF09850: DotU" amino acids 22 to 224 (203 residues), 238.8 bits, see alignment E=4.7e-75 TIGR03350: type VI secretion system peptidoglycan-associated domain" amino acids 260 to 394 (135 residues), 164.4 bits, see alignment E=1.4e-52 PF00691: OmpA" amino acids 291 to 389 (99 residues), 56.1 bits, see alignment E=4.1e-19

Best Hits

KEGG orthology group: K11892, type VI secretion system protein ImpK (inferred from 100% identity to eay:EAM_0574)

Predicted SEED Role

"Outer membrane protein ImpK/VasF, OmpA/MotB domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (413 amino acids)

>OLJFJH_01890 DotU family type VI secretion system protein (Erwinia amylovora T8)
MKQQRHSDAAQTGMNGANGHNPLVAAANPLLNAIPQIRHSVAHPDPAGLRQRLVDEIRQF
EMHCQRAGLPWEVIIGARYCLCTALDEAAALTPWGGRGVWPGNGLLVTFHNETWGGEKFF
QLLARLSQTPREQIALLELINFCLQLGFEGRYRVMDNGRSQLETIRQRLLQMIRSVRGGY
APPLSAHPEDHPVTRQLWRPLVPLWACSAVVGFLACLLYILLNWRLGDYTSPVLASIYQT
TLPEVSIHNPAPPPPAAVNLKTFLRPEIEQGLVAVRDEADRSVVTLKGDGLFTSASTEVR
GRYAEVLDRVAAAMNSVSGRILVVGYSDNVPIRSARFASNYELSLARAQSVSEQLQQHLT
QPQRVKAEGRGESNPLAPNNSAGNRARNRRVEITLLVSPGSTRAELNDMAQGN