Protein Info for OLJFJH_01450 in Erwinia amylovora T8

Annotation: molecular chaperone DnaJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 381 PF00226: DnaJ" amino acids 5 to 67 (63 residues), 97.1 bits, see alignment E=7.6e-32 TIGR02349: chaperone protein DnaJ" amino acids 5 to 351 (347 residues), 467.8 bits, see alignment E=1.4e-144 PF01556: DnaJ_C" amino acids 122 to 335 (214 residues), 182.1 bits, see alignment E=1.1e-57 PF00684: DnaJ_CXXCXGXG" amino acids 149 to 209 (61 residues), 61.9 bits, see alignment E=8.5e-21

Best Hits

Swiss-Prot: 99% identical to DNAJ_ERWT9: Chaperone protein DnaJ (dnaJ) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K03686, molecular chaperone DnaJ (inferred from 100% identity to eam:EAMY_2943)

MetaCyc: 87% identical to chaperone protein DnaJ (Escherichia coli K-12 substr. MG1655)
1.8.4.-

Predicted SEED Role

"Chaperone protein DnaJ" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (381 amino acids)

>OLJFJH_01450 molecular chaperone DnaJ (Erwinia amylovora T8)
MAKRDYYEILGVAKSADEREIKKAYKRLAMKFHPDRNQGDKESEGKFKEIKEAYEILTDA
QKRAAYDQYGHAAFEQGGMGGGGHGGFGGGGGADFSDIFGDVFGDIFGGGRRQQRAARGA
DLRYNMELSLEEAVRGVSKEIRIPTLEECGVCHGSGAKAGTKPQTCSTCHGAGQVQMRQG
FFTVQQACPACHGRGSVIKDPCNACHGHGRVEKSKTLSVKIPAGVDTGDRIRLSGEGEAG
EQGAPAGDLYVQVQVRKHHIFEREENNLYCEVPINFVMAALGGEIEVPTLDGRVNLKVPA
ETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVSLNEKQKALLRELDESFGGPSGEK
NSPRSKTFFDGVKKFFDDLTR