Protein Info for OLJFJH_01335 in Erwinia amylovora T8

Annotation: DNA polymerase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 787 PF21474: DNApolII_N" amino acids 7 to 42 (36 residues), 72.1 bits, see alignment (E = 5.2e-24) PF22587: DNApolII_insertion" amino acids 43 to 103 (61 residues), 101.8 bits, see alignment 3.3e-33 PF03104: DNA_pol_B_exo1" amino acids 201 to 299 (99 residues), 27.7 bits, see alignment E=3.5e-10 PF00136: DNA_pol_B" amino acids 380 to 442 (63 residues), 30.2 bits, see alignment 4.5e-11 amino acids 493 to 758 (266 residues), 100.2 bits, see alignment E=2.5e-32

Best Hits

Swiss-Prot: 76% identical to DPO2_ECOLI: DNA polymerase II (polB) from Escherichia coli (strain K12)

KEGG orthology group: K02336, DNA polymerase II [EC: 2.7.7.7] (inferred from 99% identity to eam:EAMY_2919)

MetaCyc: 76% identical to DNA polymerase II (Escherichia coli K-12 substr. MG1655)
3.1.11.-; DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase II (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (787 amino acids)

>OLJFJH_01335 DNA polymerase II (Erwinia amylovora T8)
VNDARAGFLLTRHWRDTPAGSEVVLWLATDSGPQQVVLPPQESVAFIPAVQQEAASRLLA
GDRHWRFAALELKDFHQRPVVGLYCKQHRQLQQLEKRLRENGITLYEADVRPPDRFLMER
FITAAVWFSGQPAGDRLVNARLKPHPDYRPPLQWVSLDIETTRHGELYCIGLEGCGQRDV
YMLGPENGDASQLDFHLEYVASRPQLLEKLNAWFARHDPDVLIGWNVVQFDLRVLQKHAD
RYGIPLMLGRGDNARLEWREHGFKPGVFFAQASGRLIIDGIEALKSAFWHFDSFSLEAVS
RQLLGEGKAINNPWQRMEEIDQRFAEDKPALAHYNLKDCELVTRIFQHTELMPFLLERAA
VNGLAVDRHGGSVAAFSHLYLPRMHRAGFVAPNLGDVAPQASPGGYVMDSRPGLYDSVLV
LDYKSLYPSIIRSFLIDPVGLVEGMAHPQEADSVSGYLGARFSRTTHCLPGIVSHIWLGR
EAAKKQGNKPLSQALKIIMNAFYGVLGTSACRFFDPRLASSITLRGHDIMLQTRELIEAE
GYDVIYGDTDSTFVWLKSAHDEAAAEHIGQRLVAKVNQWWQQHLQSEYGLQSALELEYET
HFSRFLMPTIRGAEQGSKKRYAGLTGSGDGQRMVFKGLETVRTDWTPLAQKFQQALYLKI
FTGQPYQDYIRETVRQLLDGELDEQLVYRKRLRRPLDDYQRNVPPHVRAARIADEQNQKL
GRPLQYQNGGKIRYVIATSGPEPLEARITPLDYDHYLTRQLQPVADGILPFMQDDFATLV
TGQLGLF