Protein Info for OLJFJH_00040 in Erwinia amylovora T8

Annotation: high-affinity choline transporter BetT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 676 transmembrane" amino acids 18 to 37 (20 residues), see Phobius details amino acids 57 to 76 (20 residues), see Phobius details amino acids 96 to 117 (22 residues), see Phobius details amino acids 147 to 168 (22 residues), see Phobius details amino acids 196 to 219 (24 residues), see Phobius details amino acids 234 to 255 (22 residues), see Phobius details amino acids 267 to 291 (25 residues), see Phobius details amino acids 322 to 340 (19 residues), see Phobius details amino acids 352 to 371 (20 residues), see Phobius details amino acids 405 to 425 (21 residues), see Phobius details amino acids 454 to 474 (21 residues), see Phobius details amino acids 481 to 499 (19 residues), see Phobius details PF02028: BCCT" amino acids 19 to 503 (485 residues), 596.8 bits, see alignment E=1.3e-183 TIGR00842: transporter, betaine/carnitine/choline transporter (BCCT) family" amino acids 57 to 504 (448 residues), 582 bits, see alignment E=4e-179

Best Hits

Swiss-Prot: 80% identical to BETT_ECOLI: High-affinity choline transport protein (betT) from Escherichia coli (strain K12)

KEGG orthology group: K02168, high-affinity choline transport protein (inferred from 100% identity to eam:EAMY_3737)

MetaCyc: 80% identical to choline:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-99

Predicted SEED Role

"High-affinity choline uptake protein BetT" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (676 amino acids)

>OLJFJH_00040 high-affinity choline transporter BetT (Erwinia amylovora T8)
MPATEIPEKPQKDRINPVVFYTSAVLILLFSLTTILFTEFSDRWINRTLNWVSNTFGWYY
LLAATLYIVFVIFMACSRFGSIKLGPEQSKPEFSLMSWAAMLFAAGIGIDLMFFSVAEPV
TQYMMPPEGQGQTLEAARQAMVWTLFHYGLTGWSMYALMGIALGYFSYRYGLPLTIRSAL
YPIFGKRVNGPIGHTVDIAAVLGTIFGIATTLGIGVVQLNYGLKVLFDVPEGLTAQAGLI
VLSVIMATISVTSGVNKGIRLLSELNVLLAFGLILFVLFVGDTSFLLNALVLNVGDYINR
FMGMTLDSFAFDRPAEWMNNWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVLGTLTIP
FIFTLLWLSIFGNSALNEIIHGNLALAQETLAHPERGFYSLLAQYPGFTFSASVATITGL
LFYVTSADSGSLVLGNFTSRLADINNDAPNWLRIFWSVTIGVLTMGMLMVNGVTALQKTT
VIMGLPFSFVIFFVMAGLYKSLRVEDHRRASATVNTPPVTVDRLNWKQRISRVMNYPGTT
HTRKMLDNVCRPAMEEVGRELEHRGAKVQVNELPAVDDERLNHLELLVDLADEQSFLYQI
WPQRYSVPAFTYRARSGKSDYFRLETFLLEGTQGNDLMDYTKEQIINDILDQYERHLTFL
HIHREAPGNSMPFPEV