Protein Info for OKGIIK_16965 in Rhodanobacter sp. FW510-T8

Name: thiC
Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 22 to 99 (78 residues), 100.4 bits, E=4.1e-33
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 138 to 590 (453 residues), 716.4 bits, E=5.2e-220
• PF01964: ThiC_Rad_SAM
  • amino acids 139 to 586 (448 residues), 622 bits, E=4.9e-191

Best Hits

Swiss-Prot: 82% identical to THIC_XANAC: Phosphomethylpyrimidine synthase (thiC) from Xanthomonas axonopodis pv. citri (strain 306)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 83% identity to psu:Psesu_0451)

MetaCyc: 75% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (625 amino acids)

>OKGIIK_16965 phosphomethylpyrimidine synthase ThiC (Rhodanobacter sp. FW510-T8)
MNAQPFDLARAAQELSESVVRPIPGSRKIHVPGSRPDLRVPLREIGQADTPAMFGVEKNP
PITLYDTSGPYTDPNHRVDLVAGLPPLRAAWIAERGDSEVLGGRSSEFGRRHAAAPELAA
IRFPRLPVPRRAKAGANVTQMHYARRGIVTPEMEYIAIRENQRVEALGDSALRMQHPGQS
FGASLPRLITPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLTKINANIGNSAVTSSI
AEEVEKMVWSIRWGGDTVMDLSTGKHIHETREWILRNAPVPIGTVPIYQALEKVGGVAEE
LSWELFRDTLLEQAEQGVDYFTIHAGVLLRFVPLTAKRVTGIVSRGGSIMAKWCLAHHKE
NFLYTHFEDICEIMKAYDVSFSLGDGLRPGCIADANDAAQFGELDTLGELTQLAWKHDVQ
TMIEGPGHVPMQLIKENMDRQLEKCGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNKQDVRDGIIAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRW
DDQFNLGLDPEKAQEFHDETLPKDAHKSAHFCSMCGPKFCSMKITQDVRDYAAEHGVEAF
AAIDEGMAEKAAEFRAQGAEVYRRT