Protein Info for OKGIIK_16775 in Rhodanobacter sp. FW510-T8

Annotation: DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 737 PF00580: UvrD-helicase" amino acids 13 to 275 (263 residues), 283.4 bits, see alignment E=5.7e-88 PF13245: AAA_19" amino acids 17 to 258 (242 residues), 74.2 bits, see alignment E=3.1e-24 PF13361: UvrD_C" amino acids 280 to 431 (152 residues), 157.8 bits, see alignment E=1.3e-49 amino acids 513 to 631 (119 residues), 126 bits, see alignment E=6e-40 PF13538: UvrD_C_2" amino acids 571 to 627 (57 residues), 29.9 bits, see alignment 1.1e-10 PF21196: PcrA_UvrD_tudor" amino acids 690 to 732 (43 residues), 36.5 bits, see alignment 9.1e-13

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 61% identity to tgr:Tgr7_0080)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (737 amino acids)

>OKGIIK_16775 DNA helicase (Rhodanobacter sp. FW510-T8)
MGAMDVSYLIDPLNDAQREAVCAPPGHYLVLAGAGSGKTRVLTHRIGWLTQVEHVPPWAI
LAVTFTNKAAGEMRARLENLIPGGTQGLTVGTFHGIAHRLLRRHWREAGLPEGFQILDAD
DQQRLVKRVVAGLGLDEAKFPPRQAAWTINGWKDEGKRPDAIEHRDHPVTHTFVRIYQAY
EDACRRAGLVDFAELLLRAHELWLKQPAVLEHYQQRWRHLLIDEFQDTNTLQYAWIRVLA
GATGKVFAVGDDDQSIYGWRGARVENMQQFLKDFPGARTIKLEQNYRSTSTILKAANSVI
QRNGSRLGKQLWTAGEEGERIALYAAYNEQDEARFVVERIREYIAEHGSAKDCAILYRSN
AQSRNFEEQLAQRSIGYRVYGGQRFFERAEVKDALAYLRLTANRHDDAAFERAVNTPPRG
IGDRTLDVLRRRARSENSSMWEAALSELGGGSELAGRAKNAVKAFLGMIDAMAHAFRPST
PHGEKHALTLAEQIDHAITHTGLRDFYEKDGRGNAESRVENLDELVNVASRFERTPEDVD
AGLDELAAFLSHAALEAGEGQGEAWDDCVQLMTLHSAKGLEFPLVFLVGMEEGLFPSQRS
VEDEGRLEEERRLAYVGITRARARLVVSYAESRRMHGTEMLARPSRFLAEIPAALVDEVR
PRVQVTRPLYAGRSHEVAPSLEESLPVKLGQRVSHPSFGEGVVISAEGSGAHTRLQVNFE
GAGSKWLVAAYANLTAL