Protein Info for OKGIIK_15495 in Rhodanobacter sp. FW510-T8

Name: hemC
Annotation: hydroxymethylbilane synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01379: Porphobil_deam
  • amino acids 6 to 210 (205 residues), 272.9 bits, E=1.7e-85
• TIGR00212: hydroxymethylbilane synthase
  • amino acids 6 to 297 (292 residues), 333.5 bits, E=4.7e-104
• PF03900: Porphobil_deamC
  • amino acids 226 to 295 (70 residues), 45.7 bits, E=7e-16

Best Hits

Swiss-Prot: 68% identical to HEM3_XANC5: Porphobilinogen deaminase (hemC) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K01749, hydroxymethylbilane synthase [EC: 2.5.1.61] (inferred from 68% identity to xcv:XCV0679)

MetaCyc: 56% identical to hydroxymethylbilane synthase (Escherichia coli K-12 substr. MG1655)
Hydroxymethylbilane synthase. [EC: 2.5.1.61]

Predicted SEED Role

"Porphobilinogen deaminase (EC 2.5.1.61)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 2.5.1.61)

MetaCyc Pathways

• superpathway of heme b biosynthesis from glutamate (9/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• superpathway of heme b biosynthesis from glycine (6/8 steps found)
• dipyrromethane cofactor biosynthesis (3/4 steps found)
• tetrapyrrole biosynthesis II (from glycine) (3/4 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (21/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (20/36 steps found)
• superpathway of bacteriochlorophyll a biosynthesis (5/26 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.61

Protein Sequence (305 amino acids)

>OKGIIK_15495 hydroxymethylbilane synthase (Rhodanobacter sp. FW510-T8)
MNPSTLRIATRKSALALWQAEHVAARLRATHPGLAVELVPMSTRGDEILDQPLATIGGKG
LFLKELEVAMLEGRADLAVHSLKDVPAQLEPGFALPAILPRADAADAFVSNHHDNPAALP
HGARVGTSSLRRQAQLRALRPDLELLDLRGNVGTRLAKLDAGAYDAIVLACAGLERLGLA
ARIRSRLAAPDWLPAPGQAAIAIEAREGQPGVLALLAALDDADTRLTVSAERAMNQALGG
SCTVPVGAWCTLGEHGLHLRGMVGDAASGQLLHAEASGPADQAIALGRQVAAALLAQGAA
ELLRL