Protein Info for OKGIIK_12965 in Rhodanobacter sp. FW510-T8

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 TIGR01436: glutamate--cysteine ligase" amino acids 11 to 455 (445 residues), 724.8 bits, see alignment E=1.4e-222 PF04107: GCS2" amino acids 53 to 358 (306 residues), 321.9 bits, see alignment E=2e-100

Best Hits

Swiss-Prot: 62% identical to GSH1_ARATH: Glutamate--cysteine ligase, chloroplastic (GSH1) from Arabidopsis thaliana

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 77% identity to psu:Psesu_2751)

MetaCyc: 62% identical to glutamate-cysteine ligase (Arabidopsis thaliana col)
Glutamate--cysteine ligase. [EC: 6.3.2.2]

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>OKGIIK_12965 glutamate--cysteine ligase (Rhodanobacter sp. FW510-T8)
VSIPSAVKSTPIQGRQQLADYLAAGEKPREAWRIGTEHEKFGFRTDDLRPPPFEGERGIR
ALLEGIAVRYGWEIAREGEIPVALSRGKASITLEPAGQLELSGAPLETIHQTCCEVNSHL
EEVRSVADGMGLGFLGMGFQPKWRRDEMPWMPKGRYKIMREYMPKVGSLGLDMMTRTCTV
QVNLDFSSEADMVKKFRTSLALQPIATALFADSPFTEGRPNGYLSYRSHIWTDTDPDRTG
MLDFVFANGFGYERYVDYILDVPMYFSYQDGRYIDLAGQDFKRFLAGELPTLPGTHATMK
DFADHLTTAFPEVRLKQYLEMRGADGGPWNRLCALPAFWVGLLYDDEALDAAWDLVKDFT
REERHALRDGVPKHALKLPFRNGTVRDLAEEALKIAGHGLRRRARLNRNGADESIFLEPM
IEIVQANQTPAERKLELFHSAWNGSVDPVFREFAY