Protein Info for OKGIIK_08435 in Rhodanobacter sp. FW510-T8

Name: msbA
Annotation: lipid A export permease/ATP-binding protein MsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 transmembrane" amino acids 27 to 48 (22 residues), see Phobius details amino acids 67 to 95 (29 residues), see Phobius details amino acids 169 to 189 (21 residues), see Phobius details amino acids 249 to 273 (25 residues), see Phobius details amino acids 279 to 299 (21 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 17 to 586 (570 residues), 713.1 bits, see alignment E=1.3e-218 PF00664: ABC_membrane" amino acids 33 to 299 (267 residues), 130.8 bits, see alignment E=1.2e-41 PF00005: ABC_tran" amino acids 363 to 513 (151 residues), 108.5 bits, see alignment E=6.5e-35

Best Hits

Swiss-Prot: 46% identical to MSBA_ALKEH: Lipid A export ATP-binding/permease protein MsbA (msbA) from Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 51% identity to sml:Smlt1640)

MetaCyc: 45% identical to ATP-binding lipopolysaccharide transport protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-236 [EC: 7.5.2.6]; 7.5.2.6 [EC: 7.5.2.6]

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA" in subsystem ZZ gjo need homes

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.- or 7.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (594 amino acids)

>OKGIIK_08435 lipid A export permease/ATP-binding protein MsbA (Rhodanobacter sp. FW510-T8)
MNGGKVAVWDAQTWRVYARLLGYTKRYWLMGLIALVGMVLDGGALAAFTQKLKPMIDDLF
AKKDAYLIFWMPIWIVGIFAVRGIGTFVSSYGIAYIGRNVVQAMQRDVFAAYLRLPATFF
GNEPSGQQISRVTYTSEQVASASTDAVKIMVTEGVTVIGMLYVMLSNSAYLSMALLVMVP
AIAFIATMVSRRYRLISRRIQGMMGSITGAVDESVNAHHEVRVYGGQQHEAARFDEVTHR
ARRLNLKIAATNATASTAIQSVAAVALAVLVFLATRPDVIANISPGVFVTVLTAMGALMP
SLKRLANVQANIQRGVSAAEDLFEVIDMPPEVDRGTRELVRTRGDLRFEDVRLTYPRNDF
EALRGVDLACAPGTVTALVGRSGSGKSSLVSLLPRFYEPSAGCIALDGEDYRDYTLASLR
RQIAWVGQGVVLFDDTVANNIAYGELAGASEAEIVAAAEAANAMEFIARMPQGIHTQVGQ
SGNMLSGGQRQRIAIARAILKNAPILVLDEATSALDTESEQLIQQALQRLMRDRTTLVIA
HRLSTVEGADQIAVMDQGRIIERGNHAELIALGGQYAALYRMQFHDHAAEQAGD