Protein Info for OKGIIK_07600 in Rhodanobacter sp. FW510-T8

Name: hyfB
Annotation: hydrogenase 4 subunit F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 transmembrane" amino acids 6 to 22 (17 residues), see Phobius details amino acids 29 to 50 (22 residues), see Phobius details amino acids 65 to 84 (20 residues), see Phobius details amino acids 105 to 122 (18 residues), see Phobius details amino acids 128 to 147 (20 residues), see Phobius details amino acids 159 to 181 (23 residues), see Phobius details amino acids 208 to 228 (21 residues), see Phobius details amino acids 245 to 268 (24 residues), see Phobius details amino acids 278 to 297 (20 residues), see Phobius details amino acids 316 to 336 (21 residues), see Phobius details amino acids 375 to 399 (25 residues), see Phobius details amino acids 411 to 429 (19 residues), see Phobius details amino acids 449 to 468 (20 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 124 to 416 (293 residues), 171.7 bits, see alignment E=1.1e-54

Best Hits

Swiss-Prot: 54% identical to HYFF_METI4: Hydrogenase-4 component F homolog (hyfF) from Methylacidiphilum infernorum (isolate V4)

KEGG orthology group: K12141, hydrogenase-4 component F [EC: 1.-.-.-] (inferred from 69% identity to pol:Bpro_0919)

Predicted SEED Role

"Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (483 amino acids)

>OKGIIK_07600 hydrogenase 4 subunit F (Rhodanobacter sp. FW510-T8)
VELAWVLGTPLFGAIVLAVLGARRSAAELNVVISLATLIAAAALVLRIIGHGSMTVAHEQ
FFVDPFNVFLIALTALIGFTTAVFSRPYMRIEQDHGRLNTHRLRLYHSMYQLFMAAMLVA
LSTNNMGLLWVAMEAATLSTVLLVSLYRTRASIEAAWKYFILCGVGIALALFGTILLYFA
AERLLGGEGMKALLWTHLNAVKGQLDPTVIGLAFVFLLVGYGTKVGLAPLHNWLPDAHAE
GPTPVSAVLSGLLLNVAMYAVVRCKILAAGAMHSALPGRMLMVFGLLSTVLAAFLLWRQR
DIKRLFGYSSIEHMGIITFAFGMGGPLANFAALLHMTVHSLTKSAIFFAAGHASQIAGTQ
RIDGIRGLLAVSPTVGWGLMLGSLAILGTPPFGVFTSEYMVLITAMRDHPWATPFLLVAL
AVAFAAIFFKVQPMVFGETDAQRLPHPPALVPVFVHLSIVLMLGLYIPPALAQWYRLAAS
LIG