Protein Info for OKGIIK_03875 in Rhodanobacter sp. FW510-T8

Name: pcnB
Annotation: tRNA nucleotidyltransferase/poly(A) polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 TIGR01942: poly(A) polymerase" amino acids 15 to 445 (431 residues), 496.7 bits, see alignment E=2.7e-153 PF01743: PolyA_pol" amino acids 46 to 174 (129 residues), 127.1 bits, see alignment E=8.2e-41 PF12627: PolyA_pol_RNAbd" amino acids 201 to 263 (63 residues), 56 bits, see alignment E=4.1e-19 PF12626: PolyA_pol_arg_C" amino acids 317 to 441 (125 residues), 123.4 bits, see alignment E=8e-40

Best Hits

Swiss-Prot: 55% identical to PCNB_SALTI: Poly(A) polymerase I (pcnB) from Salmonella typhi

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 62% identity to xcv:XCV1814)

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (449 amino acids)

>OKGIIK_03875 tRNA nucleotidyltransferase/poly(A) polymerase (Rhodanobacter sp. FW510-T8)
LNPESRTDPTLALRIIPRDQHVISRKNISKAALRVLYRLNEAGYAAYLVGGAVRDLLLGL
RPKDFDVATDATPDEVKKLFRNCRLIGRRFRLAHVVFGPEIIEVATFRGTGEEGGEGDRH
IVDGRIVRDNIWGSIEEDAVRRDFRVNAMYYDISDFSVRDYVGGMRDLEDRVLRLIGDPD
TRYHEDPVRMLRAARLAAKLGMSIDAAAMAPFETLGPLLADAAPARLFDESLKMFLAGNG
LKSFRMLEQSGLLKFLFPATARALRRGDEALRSLIEQGLANTDARIAEGKSVTPAFLFAV
LLWGEVRDVAQQLIGRGVEGNEAWSRAAAQVVGEQCQRVAIPRRFTFTMEEIWSLQPRFE
QVQRKRVFRLMAHPRFRAAFDFLLLRADESPALRELGRWWAHAQQLPPDVLAAALPAGGG
AVEHHAVVPAVTKPPRRRRRRSGNKSGSA