Protein Info for OKGIIK_00070 in Rhodanobacter sp. FW510-T8
Annotation: GMC family oxidoreductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to GADH1_PANCY: Gluconate 2-dehydrogenase flavoprotein from Pantoea cypripedii
KEGG orthology group: None (inferred from 72% identity to bpy:Bphyt_0227)MetaCyc: 60% identical to D-gluconate dehydrogenase dehydrogenase subunit (Pseudomonas fluorescens)
Gluconate 2-dehydrogenase (acceptor). [EC: 1.1.99.3]
Predicted SEED Role
"Gluconate 2-dehydrogenase (EC 1.1.99.3), membrane-bound, flavoprotein" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.3)
MetaCyc Pathways
- glucose degradation (oxidative) (2/5 steps found)
- L-ascorbate biosynthesis VIII (engineered pathway) (3/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.99.3
Use Curated BLAST to search for 1.1.99.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (591 amino acids)
>OKGIIK_00070 GMC family oxidoreductase (Rhodanobacter sp. FW510-T8) MTRMKPVDAVIVGFGWTGAIMGMELTETGLDVLALERGSYQDTSPDWVYPNIADELAYGI RGKLFQSLARETVTVRHAVGEAAVPYRQYGSFLLGTNVGGAGTHWNGQHYRPSPEDLEFR SHIAKRYGEKFMPADMQIQDYGVSYDELEPFLDQFEYVCGTSGRAGNLDGKLMDGGNPFE GVRKRGYPNPPVADTHSARLFADAAKALGHKPFPQPASNASRPYTNPYGVRLGPCNLCGY CERFGCFMYSKASPQTTILPVLMRRKNFSLRTGSHVTRINLDSTGKRATGVTYIDAQGQQ VEQPAALVIVAAFQMHNVRLMLLSGIGKPYDPATGTGVIGKNYAYQMMSSIGLFFDKDTA INPFIGAGAGGSQIVDDFNSDHFDHGPHGFIGGGYIIGGQTGGRPIQQLAVPPGTPAWGA AWKRAAKDSYLHTTFVGTHGSVMAYRDRYLDLDPTYKDAFGQPLLRITFDWHDNEYKMTR FVTDRALAIVEQMKPRQHSLTIRKPGDHYDIRQYQTTHTTGGVIMGTSPDTSALNRFQQS WDVHNVFVTGASAFPQNLGYNPTGLIGGLTYWSARAIRDTYLKNPGPMVQT