Protein Info for OKFHMN_21885 in Escherichia coli ECRC101

Name: hyfD
Annotation: hydrogenase 4 subunit HyfD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 30 to 50 (21 residues), see Phobius details amino acids 55 to 74 (20 residues), see Phobius details amino acids 80 to 101 (22 residues), see Phobius details amino acids 113 to 132 (20 residues), see Phobius details amino acids 138 to 156 (19 residues), see Phobius details amino acids 168 to 188 (21 residues), see Phobius details amino acids 207 to 230 (24 residues), see Phobius details amino acids 242 to 261 (20 residues), see Phobius details amino acids 268 to 289 (22 residues), see Phobius details amino acids 299 to 321 (23 residues), see Phobius details amino acids 332 to 353 (22 residues), see Phobius details amino acids 365 to 389 (25 residues), see Phobius details amino acids 409 to 432 (24 residues), see Phobius details amino acids 458 to 478 (21 residues), see Phobius details PF00662: Proton_antipo_N" amino acids 70 to 103 (34 residues), 29.3 bits, see alignment (E = 6.6e-11) PF00361: Proton_antipo_M" amino acids 133 to 413 (281 residues), 185.9 bits, see alignment E=9.9e-59

Best Hits

Swiss-Prot: 100% identical to HYFD_ECOLI: Hydrogenase-4 component D (hyfD) from Escherichia coli (strain K12)

KEGG orthology group: K12139, hydrogenase-4 component D [EC: 1.-.-.-] (inferred from 100% identity to eco:b2484)

Predicted SEED Role

"Hydrogenase-4 component D"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (479 amino acids)

>OKFHMN_21885 hydrogenase 4 subunit HyfD (Escherichia coli ECRC101)
MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLSLISAFYQADKVAVTLT
LVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLL
VFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDKAQRSALKALLITHIGSLGLY
LAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPI
SAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLL
AWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLP
RLRGVLHTLPLPGVGFCVAALAITGVPPFNGFFSKFPLFAAGFALSVEYWILLPAMILLM
IESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ