Protein Info for OKFHMN_21870 in Escherichia coli ECRC100
Name: hyfG
Annotation: hydrogenase 4 catalytic subunit HyfG
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 72% identical to HYCE_ECOLI: Formate hydrogenlyase subunit 5 (hycE) from Escherichia coli (strain K12)
KEGG orthology group: K12142, hydrogenase-4 component G [EC: 1.-.-.-] (inferred from 100% identity to ecf:ECH74115_3709)MetaCyc: 72% identical to hydrogenase 3 large subunit (Escherichia coli K-12 substr. MG1655)
Ferredoxin hydrogenase. [EC: 1.12.7.2]
Predicted SEED Role
No annotation
MetaCyc Pathways
- superpathway of fermentation (Chlamydomonas reinhardtii) (8/9 steps found)
- hydrogen production III (1/1 steps found)
- hydrogen production VIII (1/1 steps found)
- hydrogen production VI (1/2 steps found)
- superpathway of hydrogen production (1/2 steps found)
- superpathway of photosynthetic hydrogen production (3/6 steps found)
- L-glutamate degradation VII (to butanoate) (7/12 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Carotenoid biosynthesis - General
- Glyoxylate and dicarboxylate metabolism
- Insect hormone biosynthesis
- Methane metabolism
- Nucleotide sugars metabolism
- Porphyrin and chlorophyll metabolism
- Puromycin biosynthesis
- Trinitrotoluene degradation
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.-.-.-
Use Curated BLAST to search for 1.-.-.- or 1.12.7.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (571 amino acids)
>OKFHMN_21870 hydrogenase 4 catalytic subunit HyfG (Escherichia coli ECRC100) VNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWL PVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGERE IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYPFINEGNSG ARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD TGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE LVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGNIPKT LFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRV TLVDVRKRQSKTVPYKEIERYGIDRNRSPLK