Protein Info for OKFHMN_20700 in Escherichia coli ECRC101

Name: pncC
Annotation: nicotinamide-nucleotide amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02464: CinA
  • amino acids 8 to 159 (152 residues), 198.2 bits, E=3.2e-63
• TIGR00199: amidohydrolase, PncC family
  • amino acids 13 to 158 (146 residues), 212.8 bits, E=1.2e-67

Best Hits

Swiss-Prot: 99% identical to PNCC_ECOLI: Nicotinamide-nucleotide amidohydrolase PncC (pncC) from Escherichia coli (strain K12)

KEGG orthology group: K03743, (no description) (inferred from 99% identity to eco:b2700)

MetaCyc: 99% identical to NMN aminohydrolase (Escherichia coli K-12 substr. MG1655)
Nicotinamide-nucleotide amidase. [EC: 3.5.1.42]

Predicted SEED Role

"C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS"

MetaCyc Pathways

• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.42

Protein Sequence (165 amino acids)

>OKFHMN_20700 nicotinamide-nucleotide amidase (Escherichia coli ECRC101)
MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAGSSAWFERGFVTYSNEA
KAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSISGIAGPDGGSEEKPVGTV
WFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQFLQNT