Protein Info for OKFHMN_20695 in Escherichia coli ECRC101

Name: mltB
Annotation: lytic murein transglycosylase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13406: SLT_2
  • amino acids 92 to 388 (297 residues), 306.6 bits, E=8.7e-96
• TIGR02282: lytic murein transglycosylase B
  • amino acids 96 to 390 (295 residues), 431.7 bits, E=5.8e-134

Best Hits

Swiss-Prot: 99% identical to MLTB_ECOLI: Membrane-bound lytic murein transglycosylase B (mltB) from Escherichia coli (strain K12)

KEGG orthology group: K08305, membrane-bound lytic murein transglycosylase B [EC: 3.2.1.-] (inferred from 99% identity to ecc:c3255)

MetaCyc: 99% identical to membrane-bound lytic murein transglycosylase B (Escherichia coli K-12 substr. MG1655)
4.2.2.f [EC: 4.2.2.f]; 4.2.2.f [EC: 4.2.2.f]

Predicted SEED Role

"Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-)" in subsystem Peptidoglycan Biosynthesis (EC 3.2.1.-)

MetaCyc Pathways

• peptidoglycan recycling I (14/14 steps found)
• peptidoglycan recycling II (7/10 steps found)

KEGG Metabolic Maps

• Ascorbate and aldarate metabolism
• Glycosaminoglycan degradation
• Nucleotide sugars metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 4.2.2.f

Protein Sequence (396 amino acids)

>OKFHMN_20695 lytic murein transglycosylase B (Escherichia coli ECRC101)
LSYVKSVSGSDYRFLVRGVMLYHTCPWLNLLNGPLMFKRRYVTLLPLFVLLAACSSKPKP
TETETTTGTPSGGFLLEPQHNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQA
KRLDSVLRLMDNQAPTTSVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAW
QVYGVPPEIIVGIIGVETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARD
EQDDPLNLKGSFAGAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGW
VKGDQVAVMANGQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQY
WYGLPNFYTITRYNHSTHYAMAVWQLGQAVALARVQ