Protein Info for OKFHMN_20625 in Escherichia coli ECRC100

Name: ascG
Annotation: DNA-binding transcriptional regulator AscG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 337 PF00356: LacI" amino acids 4 to 49 (46 residues), 75.2 bits, see alignment 5.5e-25 PF00532: Peripla_BP_1" amino acids 61 to 308 (248 residues), 94.2 bits, see alignment E=2e-30 PF13407: Peripla_BP_4" amino acids 66 to 279 (214 residues), 68.3 bits, see alignment E=1.6e-22 PF13377: Peripla_BP_3" amino acids 172 to 331 (160 residues), 112.8 bits, see alignment E=3.8e-36

Best Hits

Swiss-Prot: 98% identical to ASCG_ECOLI: HTH-type transcriptional regulator AscG (ascG) from Escherichia coli (strain K12)

KEGG orthology group: K03487, LacI family transcriptional regulator, asc operon repressor (inferred from 98% identity to eco:b2714)

Predicted SEED Role

"AscBF operon repressor" in subsystem Beta-Glucoside Metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (337 amino acids)

>OKFHMN_20625 DNA-binding transcriptional regulator AscG (Escherichia coli ECRC100)
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKST
QTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCD
AIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVWCDHKQTSFNAVAELINAGH
QEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMI
QEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIALSR