Protein Info for OKFHMN_20495 in Escherichia coli ECRC101

Name: rpoS
Annotation: RNA polymerase sigma factor RpoS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 TIGR02394: RNA polymerase sigma factor RpoS" amino acids 31 to 311 (281 residues), 480.8 bits, see alignment E=1.6e-148 PF00140: Sigma70_r1_2" amino acids 39 to 71 (33 residues), 44.4 bits, see alignment 2.7e-15 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 73 to 300 (228 residues), 118.6 bits, see alignment E=2.1e-38 PF04542: Sigma70_r2" amino acids 77 to 146 (70 residues), 80.8 bits, see alignment E=1.1e-26 PF04539: Sigma70_r3" amino acids 157 to 231 (75 residues), 75 bits, see alignment E=8.2e-25 PF04545: Sigma70_r4" amino acids 245 to 298 (54 residues), 55.4 bits, see alignment 6.9e-19

Best Hits

Swiss-Prot: 100% identical to RPOS_ECOLI: RNA polymerase sigma factor RpoS (rpoS) from Escherichia coli (strain K12)

KEGG orthology group: K03087, RNA polymerase nonessential primary-like sigma factor (inferred from 100% identity to eco:b2741)

MetaCyc: 100% identical to RNA polymerase sigma factor RpoS (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"RNA polymerase sigma factor RpoS" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (313 amino acids)

>OKFHMN_20495 RNA polymerase sigma factor RpoS (Escherichia coli ECRC101)
MRTELRFFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEV
YFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPE
RGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEE
IAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQ
SIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREIL
QTQGLNIEALFRE