Protein Info for OKFHMN_19965 in Escherichia coli ECRC100

Name: aas
Annotation: bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 719 transmembrane" amino acids 40 to 61 (22 residues), see Phobius details amino acids 258 to 277 (20 residues), see Phobius details amino acids 415 to 438 (24 residues), see Phobius details PF01553: Acyltransferase" amino acids 15 to 137 (123 residues), 84.8 bits, see alignment E=4.8e-28 PF00501: AMP-binding" amino acids 233 to 568 (336 residues), 188.9 bits, see alignment E=1.3e-59

Best Hits

Swiss-Prot: 100% identical to AAS_ECO5E: Bifunctional protein Aas (aas) from Escherichia coli O157:H7 (strain EC4115 / EHEC)

KEGG orthology group: K05939, acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase / long-chain-fatty-acid--[acyl-carrier-protein] ligase [EC: 2.3.1.40 6.2.1.20] (inferred from 100% identity to ece:Z4154)

MetaCyc: 99% identical to fused 2-acylglycerophospho-ethanolamine acyltransferase/acyl-acyl carrier protein synthetase (Escherichia coli K-12 substr. MG1655)
RXN-5741 [EC: 6.2.1.47]; 6.2.1.- [EC: 6.2.1.47]; Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase. [EC: 6.2.1.47, 2.3.1.40]

Predicted SEED Role

"2-acylglycerophosphoethanolamine acyltransferase (EC 2.3.1.40) / Acyl-[acyl-carrier-protein] synthetase (EC 6.2.1.20)" (EC 2.3.1.40, EC 6.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.40 or 6.2.1.20 or 6.2.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (719 amino acids)

>OKFHMN_19965 bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase (Escherichia coli ECRC100)
MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGILLGLFLPVRPVFAVYTS
ISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVIFPEGRITTTGSLMKIYDG
AGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQITLHILPPTQVEMPDAPRARD
RRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFGAGKKCVEDVNFTPDSYRKLLTK
TLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAIARRRIPAMMNYTAGVKGLTSAITA
AEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLEDLKADVTTADKVWIFAHLLMPRLAQV
KQQPEEEALILFTSGSEGHPKGVVHSHKSILANVEQIKTIADFTTNDRFMSALPLFHSFG
LTVGLFTPLLTGAEVFLYPSPLHYRIVPELVYDRSCTVLFGTSTFLGHYARFANPYDFYR
LRYVVAGAEKLQESTKQLWQDKFGLRILEGYGVTECAPVVSINVPMAAKPGTVGRILPGM
DARLLSVPGIEEGGRLQLKGPNIMNGYLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVH
FDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFT
TDNELTRDKLQQYAREHGVPELAVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE