Protein Info for OKFHMN_15520 in Escherichia coli ECRC101

Name: yicI
Annotation: alpha-xylosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 772 PF13802: Gal_mutarotas_2" amino acids 55 to 218 (164 residues), 104.3 bits, see alignment E=1.6e-33 PF01055: Glyco_hydro_31_2nd" amino acids 261 to 575 (315 residues), 317.7 bits, see alignment E=1.6e-98 PF21365: Glyco_hydro_31_3rd" amino acids 584 to 668 (85 residues), 84 bits, see alignment E=1.2e-27

Best Hits

Swiss-Prot: 98% identical to XYLS_ECOLI: Alpha-xylosidase (yicI) from Escherichia coli (strain K12)

KEGG orthology group: K01811, putative family 31 glucosidase (inferred from 100% identity to ece:Z5084)

MetaCyc: 98% identical to alpha-D-xyloside xylohydrolase (Escherichia coli K-12 substr. MG1655)
RXN-12402 [EC: 3.2.1.177]; 3.2.1.177 [EC: 3.2.1.177]

Predicted SEED Role

"Alpha-xylosidase (EC 3.2.1.-)" in subsystem Xylose utilization (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 3.2.1.177

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (772 amino acids)

>OKFHMN_15520 alpha-xylosidase (Escherichia coli ECRC101)
MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRERTWQLDTPLFTLRFFSP
QEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAEFKSGNLSARVSKGEFWSL
DFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGETVYGLGERFTALVRNGQTVE
TWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSFEVGSEKVSKVQFSVESEYLEYF
VIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEATVNSFIDGMAGRNLPL
HVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKICVWINPYIGQKSPVFKEL
QEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGER
IPIDVQWFDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWG
GDCYANYESMAESLRGGLSIGLSGFGFWSHDIGGFENTALAHVYKRWCAFGLLSSHSRLH
GSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARANARGTPMMRAMMMEFPDDPA
CDYLDRQYMLGDNVMVAPVFSEAGDVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLP
VYVRDNTLLALGNNEQRPDYAWHEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTG
NTITVTGAGEAKNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL