Protein Info for OKFHMN_12755 in Escherichia coli ECRC101

Name: mdtN
Annotation: multidrug efflux transporter periplasmic adaptor subunit MdtN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR00998: efflux pump membrane protein" amino acids 10 to 340 (331 residues), 419.1 bits, see alignment E=1.1e-129 PF00529: CusB_dom_1" amino acids 25 to 339 (315 residues), 39.3 bits, see alignment E=1.3e-13 PF16576: HlyD_D23" amino acids 48 to 285 (238 residues), 52.6 bits, see alignment E=9.9e-18 PF13533: Biotin_lipoyl_2" amino acids 51 to 95 (45 residues), 48.7 bits, see alignment 1.2e-16 PF13437: HlyD_3" amino acids 210 to 293 (84 residues), 50.5 bits, see alignment E=7.4e-17

Best Hits

Swiss-Prot: 100% identical to MDTN_ECO57: Multidrug resistance protein MdtN (mdtN) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 99% identity to eco:b4082)

MetaCyc: 99% identical to putative multidrug efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-351

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (343 amino acids)

>OKFHMN_12755 multidrug efflux transporter periplasmic adaptor subunit MdtN (Escherichia coli ECRC101)
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYVSADTIDVVPEVSGRIV
ELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQIMLTQRSVDAQQFGADSVN
ATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADLNAVLLQAQSAAS
AVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGL
PKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ