Protein Info for OKFHMN_11410 in Escherichia coli ECRC100

Name: hsdR
Annotation: DEAD/DEAH box helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 810 PF04313: HSDR_N" amino acids 9 to 120 (112 residues), 36.8 bits, see alignment E=1.4e-12 PF13588: HSDR_N_2" amino acids 41 to 118 (78 residues), 32.7 bits, see alignment E=2.4e-11 PF04851: ResIII" amino acids 169 to 326 (158 residues), 158.2 bits, see alignment E=6.1e-50 PF18766: SWI2_SNF2" amino acids 173 to 358 (186 residues), 70.2 bits, see alignment E=7.4e-23 PF00270: DEAD" amino acids 174 to 328 (155 residues), 46.7 bits, see alignment E=1.1e-15 PF00271: Helicase_C" amino acids 412 to 526 (115 residues), 25 bits, see alignment E=6.8e-09 PF08463: EcoEI_R_C" amino acids 648 to 803 (156 residues), 126.2 bits, see alignment E=4.7e-40

Best Hits

Swiss-Prot: 100% identical to T1RA_ECOLX: Type I restriction enzyme EcoAI R protein (hsdR) from Escherichia coli

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 100% identity to ecs:ECs5308)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (810 amino acids)

>OKFHMN_11410 DEAD/DEAH box helicase (Escherichia coli ECRC100)
MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGKVIVRGKVAARRTVKSA
DIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAE
GECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQDYYDDGSGKSPRYYQLQAINK
TIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDF
QPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSA
SEDSAWREILDYFSSATQIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKV
VRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTMVIDERTELVARTITDYLKRT
NPMDKTIVFCNDIDHAERMRRALVNLNPEQVKKNDKYVMKITGDDEIGKAQLDNFINPKK
PYPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILDF
KKATELFADERFDGIPEKVMDTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPAPPY
QVKDTDDVGPLPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLK
EYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLT
RKERAENVRKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEI
IKTGFGDRNGYNQALSELENEIYQLPPRSA