Protein Info for OKFHMN_10180 in Escherichia coli ECRC101

Name: cdsA
Annotation: phosphatidate cytidylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 249 transmembrane" amino acids 20 to 37 (18 residues), see Phobius details amino acids 53 to 73 (21 residues), see Phobius details amino acids 83 to 105 (23 residues), see Phobius details amino acids 114 to 135 (22 residues), see Phobius details amino acids 156 to 174 (19 residues), see Phobius details amino acids 180 to 198 (19 residues), see Phobius details amino acids 228 to 246 (19 residues), see Phobius details PF01148: CTP_transf_1" amino acids 1 to 243 (243 residues), 225 bits, see alignment E=7.7e-71

Best Hits

Swiss-Prot: 100% identical to CDSA_ECO57: Phosphatidate cytidylyltransferase (cdsA) from Escherichia coli O157:H7

KEGG orthology group: K00981, phosphatidate cytidylyltransferase [EC: 2.7.7.41] (inferred from 100% identity to eco:b0175)

MetaCyc: 100% identical to CDP-diglyceride synthetase (Escherichia coli K-12 substr. MG1655)
Phosphatidate cytidylyltransferase. [EC: 2.7.7.41]; 2.7.7.41 [EC: 2.7.7.41]

Predicted SEED Role

"Phosphatidate cytidylyltransferase (EC 2.7.7.41)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.7.41)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.41

Use Curated BLAST to search for 2.7.7.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (249 amino acids)

>OKFHMN_10180 phosphatidate cytidylyltransferase (Escherichia coli ECRC101)
MLAAWEWGQLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWW
IVALLLVLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLY
VMILVWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVA
PVTLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
LLLLVFRTL