Protein Info for OKFHMN_07975 in Escherichia coli ECRC101

Name: tesA
Annotation: multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00657: Lipase_GDSL
  • amino acids 20 to 184 (165 residues), 95.1 bits, E=6.8e-31
• PF13472: Lipase_GDSL_2
  • amino acids 21 to 176 (156 residues), 103.3 bits, E=2.3e-33

Best Hits

Swiss-Prot: 100% identical to TESA_ECOLI: Thioesterase 1/protease 1/lysophospholipase L1 (tesA) from Escherichia coli (strain K12)

KEGG orthology group: K10804, acyl-CoA thioesterase I [EC: 3.1.1.5 3.1.2.-] (inferred from 100% identity to eco:b0494)

MetaCyc: 100% identical to multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (Escherichia coli K-12 substr. MG1655)
Acyl-CoA hydrolase. [EC: 3.1.2.20]; Oleoyl-[acyl-carrier-protein] hydrolase. [EC: 3.1.2.20, 3.1.2.14]; 3.1.2.14 [EC: 3.1.2.20, 3.1.2.14]; Lysophospholipase. [EC: 3.1.2.20, 3.1.2.14, 3.1.1.5]

Predicted SEED Role

"Arylesterase precursor (EC 3.1.1.2)" (EC 3.1.1.2)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (51/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (20/20 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
• cis-vaccenate biosynthesis (5/5 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• acyl-CoA hydrolysis (1/1 steps found)
• palmitate biosynthesis III (21/29 steps found)
• stearate biosynthesis IV (4/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• palmitoleate biosynthesis II (plants and bacteria) (1/2 steps found)
• phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• petroselinate biosynthesis (2/6 steps found)
• bacterial bioluminescence (3/8 steps found)
• firefly bioluminescence (2/14 steps found)
• jasmonic acid biosynthesis (4/19 steps found)
• superpathway of mycolate biosynthesis (26/239 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Limonene and pinene degradation
• Ubiquinone and menaquinone biosynthesis
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5, 3.1.2.-

Use Curated BLAST to search for 3.1.1.2 or 3.1.1.5 or 3.1.2.- or 3.1.2.14 or 3.1.2.20

Protein Sequence (197 amino acids)

>OKFHMN_07975 multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1 (Escherichia coli ECRC101)
LPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSASAAWPALLNDKWQSKTSVVNASISGD
TSQQGLARLPALLKQHQPRWVLVELGGNDGLRGFQPQQTEQTLRQILQDVKAANAEPLLM
QIRLPANYGRRYNEAFSAIYPKLAKEFDVPLLPFLMEEVYLKPQWMQDDGIHPNRDAQPF
IADWMAKQLQPLVNHDS