Protein Info for OKFHMN_06230 in Escherichia coli ECRC100

Name: rlmF
Annotation: 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 PF05971: Methyltransf_10" amino acids 3 to 296 (294 residues), 510.1 bits, see alignment E=2.1e-157

Best Hits

Swiss-Prot: 100% identical to RLMF_ECO57: Ribosomal RNA large subunit methyltransferase F (rlmF) from Escherichia coli O157:H7

KEGG orthology group: K06970, ribosomal RNA large subunit methyltransferase F [EC: 2.1.1.181] (inferred from 100% identity to ece:Z1028)

MetaCyc: 99% identical to 23S rRNA m6A1618 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11596 [EC: 2.1.1.181]

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)" (EC 2.1.1.51)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.51

Use Curated BLAST to search for 2.1.1.181 or 2.1.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (308 amino acids)

>OKFHMN_06230 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF (Escherichia coli ECRC100)
MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSVDFANPLAVKALNKALL
AHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPL
IGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLRRQKESGAIFNGIIHKNEQYD
ATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGGQQQELWCEGGEVTFIKKMIEES
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQR
RRFVNRQR