Protein Info for OKFHMN_03730 in Escherichia coli ECRC101

Name: pyrC
Annotation: dihydroorotase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00856: dihydroorotase, homodimeric type
  • amino acids 8 to 347 (340 residues), 555.6 bits, E=1.9e-171
• PF01979: Amidohydro_1
  • amino acids 15 to 307 (293 residues), 73.2 bits, E=1.2e-24

Best Hits

Swiss-Prot: 100% identical to PYRC_ECO5E: Dihydroorotase (pyrC) from Escherichia coli O157:H7 (strain EC4115 / EHEC)

KEGG orthology group: K01465, dihydroorotase [EC: 3.5.2.3] (inferred from 100% identity to eok:G2583_1321)

MetaCyc: 99% identical to dihydroorotase (Escherichia coli K-12 substr. MG1655)
Dihydroorotase. [EC: 3.5.2.3]

Predicted SEED Role

"Dihydroorotase (EC 3.5.2.3)" in subsystem De Novo Pyrimidine Synthesis (EC 3.5.2.3)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.3

Protein Sequence (348 amino acids)

>OKFHMN_03730 dihydroorotase (Escherichia coli ECRC101)
MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMPNLAPPVTTVEAAVAYR
QRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAAKLYPANATTNSSHGVTSV
DAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIESVMEPLRQRLTALKVVFEHIT
TKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRPHLYCLPILKRNIHQQALRELVA
SGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPIALGSYATVFEEMNALQHFEAFCSVNG
PQFYGLPVNDTFIELVREEHQVAESIALTDDTLVPFLAGETVRWSVKQ