Protein Info for OKFHMN_03525 in Escherichia coli ECRC101

Name: hinT
Annotation: purine nucleoside phosphoramidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 119 PF11969: DcpS_C" amino acids 5 to 106 (102 residues), 84.6 bits, see alignment E=7.2e-28 PF01230: HIT" amino acids 12 to 109 (98 residues), 109 bits, see alignment E=1.6e-35

Best Hits

Swiss-Prot: 99% identical to HINT_ECOLI: Purine nucleoside phosphoramidase (hinT) from Escherichia coli (strain K12)

KEGG orthology group: K12150, HIT-like protein HinT (inferred from 99% identity to eco:b1103)

MetaCyc: 99% identical to purine nucleoside phosphoramidase (Escherichia coli K-12 substr. MG1655)
3.9.1.-

Predicted SEED Role

"YcfF/hinT protein: a purine nucleoside phosphoramidase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (119 amino acids)

>OKFHMN_03525 purine nucleoside phosphoramidase (Escherichia coli ECRC101)
VAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIPNILIPTVNDVSAEHEQ
ALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHMHLLGGRPLGPMLAHKGL