Protein Info for OKFHMN_02495 in Escherichia coli ECRC100

Name: adeB
Annotation: hydrophobe/amphiphile efflux-1 family RND transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1027 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 339 to 358 (20 residues), see Phobius details amino acids 365 to 387 (23 residues), see Phobius details amino acids 393 to 413 (21 residues), see Phobius details amino acids 437 to 458 (22 residues), see Phobius details amino acids 469 to 495 (27 residues), see Phobius details amino acids 536 to 553 (18 residues), see Phobius details amino acids 866 to 885 (20 residues), see Phobius details amino acids 892 to 912 (21 residues), see Phobius details amino acids 918 to 942 (25 residues), see Phobius details amino acids 964 to 982 (19 residues), see Phobius details amino acids 1007 to 1025 (19 residues), see Phobius details PF00873: ACR_tran" amino acids 2 to 977 (976 residues), 1160.5 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 2 to 977 (976 residues), 1444.4 bits, see alignment E=0 PF03176: MMPL" amino acids 337 to 497 (161 residues), 31.5 bits, see alignment E=1.4e-11 PF02355: SecD_SecF" amino acids 338 to 483 (146 residues), 22.2 bits, see alignment E=1.2e-08

Best Hits

Swiss-Prot: 56% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to ecv:APECO1_450)

MetaCyc: 56% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1027 amino acids)

>OKFHMN_02495 hydrophobe/amphiphile efflux-1 family RND transporter (Escherichia coli ECRC100)
MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTIKISATYTGASAETLEN
SVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPDTAQVQNKIQQAESRLPSE
VQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLVSNVQDPLARVEGVGSLQVFGAE
YAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAGKIGALPSPNTQQLTATVRAQSRLQ
TVDQFKNIIVKSQSDSAVVRIKDVARVEMGSEDYTAIGKLNGHPSAGVAVMLSPGANALN
TATLVKDKIAEFQRNMPQGYDIAYPKDSTEFIKISVEDVIQTLFEAIVLVVCVMYLFLQN
LRATLIPALAVPVVLLGTFGVLALFGYSINTLTLFAMVLAIGLLVDDAIVVVENVERIMR
DKGLPAREATEKSMGEISGALVAIALVLSAVFLPMAFFGGSTGVIYRQFSITIISAMLLS
VVVALTLTPALCGSVLQHVPPHKKGFFGAFDRFYRRTEDKYQRGVIYVLRRAARTMGLYL
VLGGGMALMMWKLPGSFLPTEDQGEIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANT
DVIFTVDGFSFSGSGQNTGMAFVSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAM
TPPAVDGLGQSNGFTFELLANGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQL
QVDIDSNKAVSLGLSLNDVTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLG
KWFVRGSDNAMTPFSAFATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEEL
ANSLPAGTTWAWSGLSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPL
GLLGAALAAWMRDLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAA
QTRLRPIIMTSLAFIAGGNAAGDSNRLRGEQPHRHWYGHYWRYADRYVAGYFLCSSVFCT
GEAFVCR