Protein Info for OKFHMN_01535 in Escherichia coli ECRC100

Name: mddA
Annotation: L-methionine sulfoximine/L-methionine sulfone acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13420: Acetyltransf_4
  • amino acids 3 to 155 (153 residues), 49.3 bits, E=2.1e-16
• PF13302: Acetyltransf_3
  • amino acids 3 to 137 (135 residues), 44.9 bits, E=6.8e-15
• PF00583: Acetyltransf_1
  • amino acids 16 to 136 (121 residues), 75.5 bits, E=1.5e-24
• PF13673: Acetyltransf_10
  • amino acids 50 to 142 (93 residues), 36.2 bits, E=1.9e-12
• PF13508: Acetyltransf_7
  • amino acids 53 to 137 (85 residues), 49.3 bits, E=1.9e-16
• PF12746: GNAT_acetyltran
  • amino acids 74 to 139 (66 residues), 28.5 bits, E=4.2e-10
• PF08445: FR47
  • amino acids 84 to 139 (56 residues), 34.9 bits, E=4.4e-12

Best Hits

Swiss-Prot: 99% identical to MNAT_ECOLI: L-amino acid N-acyltransferase MnaT (mnaT) from Escherichia coli (strain K12)

KEGG orthology group: K03823, phosphinothricin acetyltransferase [EC: 2.3.1.183] (inferred from 99% identity to eco:b1448)

MetaCyc: 99% identical to L-amino acid N-acyltransferase (Escherichia coli K-12 substr. MG1655)
Amino-acid N-acetyltransferase. [EC: 2.3.1.1]

Predicted SEED Role

"Putative acetyltransferase"

MetaCyc Pathways

• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.1

Use Curated BLAST to search for 2.3.1.1 or 2.3.1.183

Protein Sequence (172 amino acids)

>OKFHMN_01535 L-methionine sulfoximine/L-methionine sulfone acetyltransferase (Escherichia coli ECRC100)
MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFEARTIAGYPVLVSEEDG
VVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVAGI
ESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQLQLDERTEPDAIG