Protein Info for OHPLBJKB_04253 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Anaerobic sulfatase-maturating enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 TIGR03942: anaerobic sulfatase maturase" amino acids 11 to 390 (380 residues), 518.2 bits, see alignment E=1.2e-159 PF04055: Radical_SAM" amino acids 19 to 187 (169 residues), 64.2 bits, see alignment E=1.7e-21 TIGR04085: radical SAM additional 4Fe4S-binding SPASM domain" amino acids 282 to 371 (90 residues), 65.4 bits, see alignment E=5e-22 PF13186: SPASM" amino acids 283 to 343 (61 residues), 46.1 bits, see alignment E=4.8e-16

Best Hits

Swiss-Prot: 98% identical to ASLB_ECOLI: Anaerobic sulfatase-maturating enzyme homolog AslB (aslB) from Escherichia coli (strain K12)

KEGG orthology group: K06871, (no description) (inferred from 100% identity to ecl:EcolC_4206)

Predicted SEED Role

"Putative arylsulfatase regulatory protein" in subsystem Alkanesulfonate assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (411 amino acids)

>OHPLBJKB_04253 Anaerobic sulfatase-maturating enzyme (Escherichia coli HS(pFamp)R (ATCC 700891))
MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTHMDDDTLEAYIRHYIAA
SELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISNSFQTNGVLLDDEWCAFLA
EHHFLVGLSLDGPPEIHNQYRVTKGGRPTHKLVMRALTLLQKHHVDYNVLVCVNRTSAQQ
PLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLHAPGDIQGELTEWSVRPEEFGEF
LVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVCHHQPTCGRSVIVEHNGDVYACDHY
VYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQLPAQCRSCNVLKACWGGCPKHRFMLD
ASGKPGLNYLCAGYQRYFRHLPPYLKAMADLLAHGRPASDIMHAHLLVVSK