Protein Info for OHPLBJKB_04061 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: 2-iminoacetate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02351: thiazole biosynthesis protein ThiH
  • amino acids 3 to 368 (366 residues), 533.4 bits, E=1.3e-164
• PF04055: Radical_SAM
  • amino acids 81 to 233 (153 residues), 52.7 bits, E=6e-18
• PF06968: BATS
  • amino acids 258 to 360 (103 residues), 49.9 bits, E=3.1e-17

Best Hits

Swiss-Prot: 99% identical to THIH_ECOLI: 2-iminoacetate synthase (thiH) from Escherichia coli (strain K12)

KEGG orthology group: K03150, thiamine biosynthesis ThiH (inferred from 99% identity to eco:b3990)

MetaCyc: 99% identical to 2-iminoacetate synthase (Escherichia coli K-12 substr. MG1655)
RXN-11319 [EC: 4.1.99.19]

Predicted SEED Role

"2-iminoacetate synthase (ThiH) (EC 4.1.99.19)" (EC 4.1.99.19)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.19

Protein Sequence (377 amino acids)

>OHPLBJKB_04061 2-iminoacetate synthase (Escherichia coli HS(pFamp)R (ATCC 700891))
MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMALLSPAASGYLEQLAQR
AQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDEADIARESAAIREM
GFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQPLAETEYAELKQLGLDGVMV
YQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
EHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMDERQLVQTICAFRLLAPEIELSLST
RESPWFRDRVIPLAINNVSAFSKTQPGGYADNHPELEQFSPHDDRRPEAVAAALTAQGLQ
PVWKDWDSYLGRASQRL