Protein Info for OHPLBJKB_04034 in Escherichia coli HS(pFamp)R (ATCC 700891)
Annotation: Isocitrate dehydrogenase kinase/phosphatase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to ACEK_ECO8A: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Escherichia coli O8 (strain IAI1)
KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 100% identity to eco:b4016)MetaCyc: 100% identical to isocitrate dehydrogenase kinase/phosphatase (Escherichia coli K-12 substr. MG1655)
3.1.3.-; [Isocitrate dehydrogenase (NADP(+))] kinase. [EC: 2.7.11.5]
Predicted SEED Role
"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)
KEGG Metabolic Maps
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Ether lipid metabolism
- Fructose and mannose metabolism
- Lipopolysaccharide biosynthesis
- Nicotinate and nicotinamide metabolism
- Riboflavin metabolism
- Sphingolipid metabolism
- Thiamine metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.1.3.-
Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (578 amino acids)
>OHPLBJKB_04034 Isocitrate dehydrogenase kinase/phosphatase (Escherichia coli HS(pFamp)R (ATCC 700891)) MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGL VVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER LFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIIRHL TETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE YLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLV KEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIERRMV PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF RADYWRALQNRIREGHVEDVYAYRRRQRFSVRYGEMLF