Protein Info for OHPLBJKB_03957 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Multidrug resistance protein MdtN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR00998: efflux pump membrane protein" amino acids 10 to 340 (331 residues), 418.1 bits, see alignment E=2.2e-129 PF00529: CusB_dom_1" amino acids 26 to 339 (314 residues), 39.3 bits, see alignment E=1.3e-13 PF16576: HlyD_D23" amino acids 46 to 285 (240 residues), 54.4 bits, see alignment E=2.7e-18 PF13533: Biotin_lipoyl_2" amino acids 51 to 96 (46 residues), 52.8 bits, see alignment 6.5e-18 PF13437: HlyD_3" amino acids 210 to 293 (84 residues), 48.5 bits, see alignment E=3.3e-16

Best Hits

Swiss-Prot: 100% identical to MDTN_ECOLI: Multidrug resistance protein MdtN (mdtN) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b4082)

MetaCyc: 100% identical to putative multidrug efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-351

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (343 amino acids)

>OHPLBJKB_03957 Multidrug resistance protein MdtN (Escherichia coli HS(pFamp)R (ATCC 700891))
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIV
ELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVN
ATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADLNAVLLQAQSAAS
AVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASTMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGL
PKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ