Protein Info for OHPLBJKB_03925 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Alpha-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF02056: Glyco_hydro_4" amino acids 6 to 188 (183 residues), 272.9 bits, see alignment E=1.1e-85 PF11975: Glyco_hydro_4C" amino acids 198 to 421 (224 residues), 202.5 bits, see alignment E=8.5e-64

Best Hits

Swiss-Prot: 100% identical to AGAL_ECOLI: Alpha-galactosidase (melA) from Escherichia coli (strain K12)

KEGG orthology group: K07406, alpha-galactosidase [EC: 3.2.1.22] (inferred from 100% identity to eco:b4119)

MetaCyc: 100% identical to alpha-galactosidase (Escherichia coli K-12 substr. MG1655)
Alpha-galactosidase. [EC: 3.2.1.22]; RXN-17754 [EC: 3.2.1.22]

Predicted SEED Role

"Alpha-galactosidase (EC 3.2.1.22)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Melibiose Utilization (EC 3.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (451 amino acids)

>OHPLBJKB_03925 Alpha-galactosidase (Escherichia coli HS(pFamp)R (ATCC 700891))
MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
GASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
MRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
EELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCV
EQLANWHKELEEYKNASRIDIKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGC
CVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
TAAVLGIDEIYALVDDLIAAHGDWLPGWLHR