Protein Info for OHPLBJKB_03919 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Sensor histidine kinase DcuS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 543 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details amino acids 182 to 204 (23 residues), see Phobius details PF17203: sCache_3_2" amino acids 47 to 179 (133 residues), 79.9 bits, see alignment E=7.2e-26 PF00989: PAS" amino acids 222 to 323 (102 residues), 45.5 bits, see alignment E=2.4e-15 PF13596: PAS_10" amino acids 226 to 323 (98 residues), 29.5 bits, see alignment E=3.1e-10 PF14689: SPOB_a" amino acids 340 to 384 (45 residues), 33.1 bits, see alignment 1.1e-11 PF14501: HATPase_c_5" amino acids 434 to 529 (96 residues), 33.7 bits, see alignment E=1e-11 PF02518: HATPase_c" amino acids 434 to 535 (102 residues), 79.4 bits, see alignment E=1e-25

Best Hits

Swiss-Prot: 100% identical to DCUS_ECO57: Sensor histidine kinase DcuS (dcuS) from Escherichia coli O157:H7

KEGG orthology group: K07701, two-component system, CitB family, sensor histidine kinase DcuS [EC: 2.7.13.3] (inferred from 100% identity to eco:b4125)

MetaCyc: 100% identical to sensor histidine kinase DcuS (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Fumarate respiration sensor kinase protein DcuS"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (543 amino acids)

>OHPLBJKB_03919 Sensor histidine kinase DcuS (Escherichia coli HS(pFamp)R (ATCC 700891))
MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVA
RTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPF
KGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQIGVVAIGLELSRVTQQINDSR
WSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDD
RGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRL
LLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILG
LLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGFLISKINRATDLGHTLILNSE
SLLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGI
APDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGER
SNR