Protein Info for OHPLBJKB_03782 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Ornithine carbamoyltransferase subunit I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to OTC_ECOLU: Ornithine carbamoyltransferase (argI) from Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)

KEGG orthology group: K00611, ornithine carbamoyltransferase [EC: 2.1.3.3] (inferred from 99% identity to eco:b4254)

MetaCyc: 99% identical to ornithine carbamoyltransferase ArgI (Escherichia coli K-12 substr. MG1655)
Ornithine carbamoyltransferase. [EC: 2.1.3.3]

Predicted SEED Role

"Ornithine carbamoyltransferase (EC 2.1.3.3)" in subsystem Arginine Biosynthesis extended or Arginine Deiminase Pathway or Arginine and Ornithine Degradation (EC 2.1.3.3)

MetaCyc Pathways

superpathway of arginine and polyamine biosynthesis (16/17 steps found)
L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
L-citrulline degradation (3/3 steps found)
L-citrulline biosynthesis (6/8 steps found)
L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
L-arginine degradation XIII (reductive Stickland reaction) (3/5 steps found)
urea cycle (3/5 steps found)
superpathway of L-citrulline metabolism (8/12 steps found)
L-arginine biosynthesis IV (archaea) (4/9 steps found)
L-arginine degradation XIV (oxidative Stickland reaction) (1/6 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (334 amino acids)

>OHPLBJKB_03782 Ornithine carbamoyltransferase subunit I (Escherichia coli HS(pFamp)R (ATCC 700891))
MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLTGKNIALIFEKDSTRTR
CSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGYGQEIVETLAEY
AGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALT
GLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTEDVAKGVEGADFIYTDVWVSMGEA
KEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLPAFHDDQTTLGKKMAEEFGLHGGME
VTDEVFESAASIVFDQAENRMHTIKAVMVATLSK